脐血单个核细胞移植对大鼠脑梗死后神经功能的影响

Influence of neuroplasticity of rat cerebral hemorrhage by Breviscapine

目的探讨脐血单个核细胞移植(Mesnchymal Stem Cells,MSCs)对大鼠脑梗死后神经功能的影响。方法将90只实验大鼠随机分成MSCs组、模型组;制备脑梗死模型;免疫组化检测Nestin、Shank1表达;电镜观察神经元超微结构并对神经突触进行定量分析;实验大鼠神经功能检测。结果 MSCs组Shank1及Nestin蛋白阳性表达升高(P<0.01)。MSCs组神经元及突触结构正常,突触数量、界面曲率及突触后致密区增大,突触间隙变窄(P<0.01)。模型组神经元形态异常、突触溶解、胞质空化、线粒体肿胀、细胞器减少。神经功能评分,神经行为学检测,梗死组不对称分值升高,MSCs组不对称分值较明显下降(P<0.01)。结论脐血单个核细胞移植可促进大鼠脑梗死后神经功能恢复。

Objective To study the influence of neural plasticity of rat cerebral hemorrhage model after injection of mesenchymal stem ceils. Methods The models were made by brain solid positioner. 90 rats were randomly divided into model group and MSCs groups. The uhrastructure of nervous tissue was observed by transmission electron microscope and study the nerve synapse quantificationally. The expression of Shankl and Nestin protein were detected by immunohistochemistry. Evaluate the neural function of rats. Results The expression of Shankl and nestin protein increased in the MSCs group. It is significant in statistics compared with the model group（P 〈0. 01 ）. The number of nerve synapse and cellular organ were destroyed in the model group. The synapse structure was normal in the MSCs group. The curvature of synaptic boundary magnified and dense area of postsynatic thicken. The gap of cynapse narrowed obviously. It is significant in statistics compared with the model group（P 〈 0. 01 ）. The neurological scores of MSCs group was significantly decreasedly. It is significant in statistics compared with the model group. Conclusion The mesenchymal stem ceils can strengthen the plasticity and protect the function of nerve.