摘要
目的 探讨白癜风患者血清中干扰素（IFN-γ）-γ2、白细胞介素（IL）-4、转化生子因子α（TGF-α）、肿瘤坏死因子-β（TNF-β）表达水平和病变组织中切除修复交叉互补蛋白（ERCC1）、γ-H2AX蛋白表达变化与白癜风分期的关系.方法 酶联免疫吸附试验（ELISA）检测不同分期白癜风患者血清中IFN-γ、IL-4、TGF-α、TNF-β表达水平的变化,蛋白印记（Western blot）检测病变组织中ERCC1和γ-H2AX蛋白表达变化.结果 稳定期和进展期白癜风患者与对照组相比,患者血清中IFN-γ和TGF-β表达水平降低（F=37.48,F=42.41,P＜0.05）,IL-4和TNF-β表达水平显著增加（F=56.37,F=77.19,P＜0.05）.进展期白癜风患者与对照组相比,患者血清的IFN-γ/IL-4比值降低（F=46.27,P＜0.05）;稳定期和进展期白癜风患者与对照组相比,病变组织中修复交叉互补蛋白（ERCC1）蛋白水平降低（F =45.71,P＜0.05）,而γ-H2AX蛋白水平显著增高（F =37.96,P＜0.05）.结论 IFN-y、IL-4、TGF-α和TNF-β参与白癜风的发病过程,ERCC1和γ-H2AX蛋白与白癜风病理损伤密切相关.
Objective To explore the relationship between the expression of interferon-γ（IFN-γ）,interleukin （IL）-4,transforming growth factor-α （TGF-α） and tumor necrosis factor-β（TNF-β） in serum and excision repair cross complementing-1 （ERCC1） and γ-H2AX protein in pathological tissues of vitiligo patients.Methods Enzyme-linked immunosorbent assay （ELISA）was used to detect the expression of IFN-γ,IL-4,TGF-α and TNF-β in serum of vitiligo patients with different phases.Western blot was used to detect the ERCC1 and γ-H2AX protein in pathological tissues of vitiligo patients.Results Compared with control,the expression of IFN-γ and TGF-β was decreased（F =37.48,F =42.41,P 〈 0.05）,while the expression of IL-4 and TNF-β was increased in serum of vitiligo patients in stable and progressive phase（F =56.37,F =77.19,P 〈 0.05）.Compared with control,the ratio of IFN-γ/IL-4 decreased in vitiligo patients in progressive phase （F =46.27,P 〈 0.05）.Compared with control,the expression of excision repair cross complementing 1 ERCC1 protein was significantly decreased（F =45.71,P 〈 0.05）,while γ-H2AX protein was significantly increased in pathological tissues of vitiligo patients in stable and progressive phase （F =37.96,P 〈 0.05）.Conclusion IFN-γ,IL-4,TGF-α and TNF-β might take part in the pathogenesis of vitiligo.ERCC1 and γ-H2AX protein were associated with the pathological damage of vitiligo.
出处
《国际免疫学杂志》
CAS
2015年第3期239-241,共3页
International Journal of Immunology
