CXCR4抑制剂对大鼠肺型氧中毒肺损伤的干预作用

Intervention of AMD3100 in lung tissues of rats during pulmonary oxygen intoxication

目的研究趋化因子受体4(chemokine receptor 4,CXCR4)特异性拮抗剂AMD3100在大鼠肺型氧中毒(pulmonary oxygen intoxication)肺组织损伤中的干预作用。方法 40只雄性SD大鼠随机分为常压空气PBS组、常压空气抑制剂组、纯氧暴露PBS组及纯氧暴露抑制剂组,每组10只。纯氧暴露PBS组及纯氧暴露抑制剂组利用0.23 MPa纯氧加压暴露,出舱后通过HE染色切片观察大鼠肺组织病理改变,ELISA法测定炎症因子TNF-α及IL-1β含量变化,应用Western印迹方法研究大鼠肺组织caspase-3含量变化。结果纯氧暴露抑制剂组大鼠肺组织淤血、水肿、出血与纯氧暴露PBS组相比程度较轻。纯氧暴露抑制剂组TNF-α、IL-1β及活化的caspase-3含量均低于纯氧暴露PBS组。结论应用AMD3100阻断CXCR4来减轻肺型氧中毒肺损伤是有效的。

Objective To investigate the intervention of chemokine receptor 4 （CXCR4） antagonist AMD3100 in lung tissues of rats during pulmonary oxygen intoxication. Methods Forty SD rats were randomly divided into 4 groups： normal pressure air PBS group, normal pressure air antagonist group , oxygen exposure PBS group and oxygen exposure antagonist group, each consisting of 10 animals. The last two groups were compressed to 0.23 MPa at an exponential rate of 0.1 MPa/ min by pure oxygen. Pathological changes of lung tissues were observed by hematoxylin eosin.stain. Changes in TNF-α and IL-1β expression levels in the lung tissues of rats were detected by ELISA. Changes in CXCR4 expression levels were observed by Western blotting. Results Pathological examination indicated that edema and hemorrhage in the alveolar and pulmonary interstitial tissue of oxygen exposure antagonist group were lighter than in oxygen exposure PBS group. The levels of TNF-α, IL-1β and cleaved-easpase-3 in the lung tissues of the oxygen exposure antagonist group were lower than in oxygen exposure PBS group. Conclusion Blocking CXCR4 with AMD3100 can effectively alleviate lung injury during pulmonary oxygen intoxication.