多参量光声成像及其在生物医学领域的应用

Multi-parameter photoacoustic imaging and its application in biomedicine

光声成像兼具声学成像和光学成像两者的优点,因而成为近十年来发展最迅速的生物医学成像技术之一.本文介绍了光声成像的特点及其相对于广泛应用的光学成像技术和声学成像技术的优点;其次,解释了光声成像的成像原理,在此基础上介绍了光声断层成像和光声显微镜这两种典型的光声成像方案,并介绍了它们的技术特点;然后,介绍了光声成像对生物组织的生化特性、组织力学特性、血液流速分布、温度分布参数、微结构特性等多信息参量的提取能力,及其在生物系统的结构成像、功能成像、代谢成像、分子成像、基因成像等多领域的应用;最后,展望了光声成像在生物医学领域的应用潜力并讨论了其局限性.

Photoacoustic imaging is a hybrid imaging technique based on the photoacoustic effect. As a non-invasive and non-ionizing modality, photoacoustic imaging takes the both merits of the conventional acoustic imaging and optical imaging. Firstly, the contrast of photoacoustic imaging primarily depends on the optical absorption. The unique optical spectra of atoms and molecules makes optical methods to be a widely used modality to probe the molecular and chemical information of biological tissue. Therefore, photoacoustic imaging has its inherent advantage in high-contrast functional and physiological imaging of biological tissue, as well as the optical imaging method. Secondly, photoacoustic imaging has the high spatial resolution in deep tissue in comparison with the pure optical imaging method. Since the strongly optical scattering in biological tissue, pure optical imaging method is difficult to obtain the high-resolution image in the tissue deeper than ～1 ram. Whereas, acoustic wave suffers much less from scattering than optical wave, the acoustic scattering coefficient is about 2-3 orders of magnitude less than the optical scattering coefficient. Photoacoustic imaging can achieve a fine resolution in deep tissue, which equivalent to 1/200 of the imaging depth. Thirdly, non-ionizing radiation used for photoacoustic imaging is much safer than X-ray. Moreover, the low-temperature rises make photoacoustic imaging be safely used in live tissue. A laser-induced temperature rise of 1 mK yields an initial pressure of ～800 Pa in soft tissue. Such a sound pressure level has reached the sensitivities of typical ultrasonic transducers. Fourthly, photoacoustic imaging has the ability of extracting multiple contrasts, including biochemical parameter, biomechanical parameter, blood velocity distribution, tissue temperature, and microstructure information. Photoacoustic imaging can capture more specific and reliable information about the tissue structure, function, metabolism, molecule, and gene. As a result, photoacoustic imaging has become one of the fastest growing biomedical imaging techniques in the past decade. In this review, we will explain photoacoustic effect and the principle of photoacoustic imaging. Then, we introduce the two classical photoacoustic imaging schemes, including photoacoustic tomography and photoacoustic microscopy. Their main specifications, such as resolution, are also presents. We review the ability of photoacoustic imaging in extracting multiple contrasts and discuss their biomedicine applications. In addition, we also introduce the remarkable breakthroughs in super-resolution photoacoustic imaging. Finally, we look the further development and the limitations of photoacoustic imaging.