自身免疫性慢性荨麻疹患者外周血单个核细胞TLR7的表达和意义

Expression and significance of TLR7 in peripheral blood mononuclear cells of patients with autoimmune chronic urticaria

目的检测自身免疫性慢性荨麻疹(AIU)患者外周血单个核细胞(PBMCs)中Toll样受体7(TLR7)mRNA与蛋白的表达,探讨AIU的发病机制。方法采集AIU患者30例与正常对照组30例外周血,淋巴细胞分离液分离PBMCs,提取RNA,采用实时荧光定量逆转录PCR检测TLR7 mRNA表达;采用免疫荧光染色、流式细胞仪检测PBMCs中TLR7蛋白的表达。结果与正常对照组相比,AIU患者PBMCs中TLR7 mRNA的表达差异无统计学意义(P>0.05);蛋白表达增强,差异有统计学意义(P<0.05)。结论 AIU患者PBMCs中TLR7蛋白表达增强,TLR7可能在AIU的发病中起作用。

Objective To investigate the expression of Toll- like receptor 7 （TLR7） mRNA and protein in peripheral blood mononuclear cells （PBMCs） of patients with autoimmune chronic urticaria （AIU）, and to explore the immunological mechanism of AIU. Methods PBMCs from 30 patients with AIU and 30 normal controls were separated by Fico[1 - Hypaque solution. The expressions of TLR7 mRNA and protein were detected respectively by real - time quantitative reverse transcription polymerase chain reaction and by flow - cytometrie analysis. Results As compared to normal controls, the expression of TLR7 protein in PBMCs of patients with AIU significantly increased （P〈0.05）, but no difference was found in the expression of TLR7 mRNA （P 〉 0.05）. Conclusions The expression of TLR7 protein increases in patients with AIU, and the findings suggest that TLR7 may play a role in the pathogenesis of AIU.