肾癌患者的血清氨基酸含量及其临床意义

Serum amino acids content and its clinical significance in kidney cancer patients

目的探讨肾癌患者术前血清与健康人血清的氨基酸差异及其临床意义。方法收集病理确诊的肾癌患者140例(病例组)术前血清及140例健康人(对照组)的血清标本,应用高效液相色谱(HPLC)代谢组学法检测两组标本中血清游离氨基酸的含量,并进行比较分析。结果与对照组相比,病例组肾癌患者血清存在明显的氨基酸代谢异常,其中天冬氨酸、丝氨酸、甘氨酸、组氨酸、精氨酸、苏氨酸、丙氨酸、脯氨酸、胱氨酸、酪氨酸、缬氨酸、蛋氨酸、异亮氨酸、亮氨酸、苯丙氨酸和赖氨酸含量呈明显下降趋势,两组差异具有统计学意义(P<0.05)。结论肾癌患者血清氨基酸含量较正常健康人整体下降,此结果对进一步研究其发生机理以及临床特异性标记物的研究有重要意义。

Objective To investigate the differences in serum amino acids in the healthy people and patients with renal cell carcinoma（RCC） and to explore the clinical significance.Methods 140 samples of serum amino acids in RCC patients and 140 samples of serum amino acids in healthy people were collected and analyzed by the HPLC to detect and compare their content of free amino acids in serum.Results Compared with the healthy people in the control group,patients with renal cell carcinoma（RCC） had obvious disturbance of amino acid metabolism in their serum,with obvious decline in the plasma aspartate,the serine,the glycine,the histidine,the arginine,the threonine,the alanine,the proline,the tyrosine,the cystine,the valine,the methionine,the lysine,the isoleucine,the leucine,and the phenylalanine,and the statistical difference was significant（P〈0.05）.Conclusion The detection of specific amino acids in patients with kidney cancer was proved significant for the study on cancer development mechanism and its clinical specific biomarkers.