肾透明细胞癌患者血清中miR-21的表达及其临床意义

The changes of expression levels of miR-21 in the serum of patients with renal cell carcinoma

目的探讨肾癌患者与健康人血清中miR-21的表达差异及其切除肿瘤组织后血清中的表达变化,为后期深入性研究奠定理论基础。方法收集新疆医科大学第一附属医院泌尿外科新诊断肾癌患者30例术前血清样本(cRCC组),并于术后1个月收集其血清,另选择30例健康志愿者血清作为对照组,采用实时荧光定量PCR技术检测cRCC组术前、术后和对照组血清中miR-21的表达。结果 cRCC组术前、术后及对照组血清中miR-21的表达水平差异有统计学意义(Kruskal-wallis H秩和检验,H≈χ2,P=0.001),cRCC组术前血清中miR-21表达水平是对照组的12.99倍(Z=-3.252,P=0.001),术后的表达水平是对照组的1.03倍(Z=-0.103,P=0.918),cRCC组术后血清中miR-21表达水平较术前明显下降(Z=-3.349,P=0.001)。结论肾癌患者血清中miR-21明显高表达,在切除肿瘤组织后其表达水平明显下降,提示其与肾肿瘤密切相关,是肾癌潜在分子标记物。

Objective To investigate the expression levels of miR-21 in the serum of patients with renal cell carcinoma,and to lay a theoretical foundation for the in-depth study in the future.Methods 30 serum samples of patients with renal cell carcinoma was collected before surgery,and the blood serum samples were collected again after surgery a month later.Moreover,the study includes 30 healthy controls.The expression levels of miR-21 in the serum of samples from three groups were compared by using the real-time PCR.Results miR-21 expression in serum have statistical significance amang renal clear cell carcinoma,post-operation and normal group（Kruskal-wallis H rank sum test H,H≈χ^2= 14.882,P = 0.001）.compared with the normal group,miR-21 expression in renal cell carcinoma is 12.99 times（Z =-3.252,P = 0.001）,and in post-operation is 1.03 times（Z =-0.103 P= 0.918）.Conclusion The expression levels of miR-21 in the serum in patients with renal cell carcinoma is significantly increased,while it decreased significantly after resection.The outcome suggests that the expression levels of miR-21 in the serum is closely related with renal cell carcinoma.Therefore,miR-21 can be regarded as a potential molecular marker in renal cell carcinoma.