肉苁蓉苯乙醇苷类成分对BSA致大鼠肝纤维化影响的实验研究

An experimental study of Cistanche phenylethanoid glycosides on liver fibrosis induced by BSA in rats

目的观察肉苁蓉苯乙醇苷类成分(CPh Gs)对牛血清白蛋白(BSA)致大鼠肝纤维化的防治作用。方法将SD大鼠随机分为6组,每组12只,即正常对照组、肝纤维化模型组(模型组)、复方鳖甲软肝片阳性对照组(600 mg/kg)及CPh Gs高剂量组(500 mg/kg)、中剂量组(250 mg/kg)、低剂量组(125 mg/kg);采用静脉注射BSA诱导建立大鼠肝纤维化模型;造模同时,灌胃给予大鼠CPh Gs提取物,连续12 w,末次给药后腹主动脉采血,取出肝脏和脾脏。计算肝脾指数,检测大鼠血清肝功能指标及肝脏病理组织学变化。结果与正常对照组比较,模型组大鼠肝脏指数明显增加,血清AST含量明显增加。与模型组比较,复方鳖甲软肝片阳性对照组及CPh Gs不同剂量组大鼠肝脏指数均显著降低,CPh Gs不同剂量组大鼠脾脏指数明显下降,CPh Gs不同剂量组大鼠血清ALT、AST含量显著下降。CPh Gs可使BSA诱导的大鼠肝脏纤维增生明显减轻。病理结果显示,正常对照组大鼠肝组织未见异常,CPh Gs不同剂量组治疗效果显著,形态基本上接近于正常对照组。结论肉苁蓉苯乙醇苷类成分具有保护肝细胞和防治肝纤维化的作用。

Objective To observe prevention and treatment effect of Cistanche phenylethanoid glycosides to bovine serum albumin（BSA） induced liver fibrosis in rats.Methods Using intravenous injection of BSA immunity induced rat liver fibrosis model.Meanwhile,orally administered to rats CPhGs extract for 12 weeks,after the last administration of abdominal aortic blood,remove the liver and spleen.Calculation of liver indices and spleen indices,detection of ALT,AST in rat serum and liver pathological histology changes.Results Compared with the normal group,model group organize index significantly increased,serum AST was significantly increased.Compared with the model group,liver index of Biejiaruangan tablets group and CPhGs different dose groups were significantly decreased,spleen index of CPhGs different doses significantly decreased,serum ALT and AST of CPhGs different dose groups were significantly decline.CPhGs can reduce rat liver fibrosis induced by BSA.Conclusion CPhGs can protect liver cells,prevent and treat of liver fibrosis in rats.