小茴香挥发性成分GC指纹图谱研究

On GC fingerprint analysis of fennel volatile constituents

目的建立小茴香挥发性成分气相色谱指纹图谱分析方法,对挥发油成分进行分析,为控制小茴香挥发油质量提供依据。方法采用水蒸气蒸馏法提取小茴香挥发油,对10个产地小茴香进行气相色谱分析,用气相色谱建立挥发油指纹图谱分析法,并用GC-MS对指纹峰进行鉴别。结果指纹图谱中共有10个共有峰,其中8个成分得到鉴定,所建立指纹图谱分析方法学各项目符合规定,各批样品相似度>0.95。结论所建立指纹图谱方法分析简便易行,为更好控制小茴香的质量提供了新的方法。

Objective To establish a GC fingerprint analysis method for the quality control of fennel.Methods Fennel oil was extracted by steam distillation method.The fingerprint analysis of volatile oil was conducted by GC and the fingerprint peaks were identified with GC-MS.Results The batch of fennel were analyzed by GC,and ten common peaks were shown in the fingerprints,nine of which were identified.The established fingerprint analysis method was validated.The similarities of samples were over 0.95.Conclusion The fingerprint analysis method is simple,practical and suitable for the quality control of fennel.