市售新疆紫草药材的质量考察

Investigation on quality of Arnebia euchroma(Royle) Johnst in Xinjiang medicinal materials markets

目的考察新疆部分地区市场销售的新疆紫草药材的质量。方法收集新疆不同地区市场上销售的新疆紫草药材10批,采用紫外分光光度法（药典法）测定羟基萘醌总色素含量,反相高效液相色谱法测定紫草中左旋紫草素、乙酰紫草素、去氧紫草素、β,β＇-二甲基丙烯酰紫草素的含量。色谱条件：色谱柱ScienhomeKromasilC18（4.6mm×200mm,5μm）,流动相为乙腈-0.3%磷酸,采用梯度洗脱,流速1.0mL/min,柱温35℃,检验波长274,洗脱时间50min。结果紫外分光光度法测得10批药材中羟基萘醌总色素含量为0.60～17.30mg/g,高效液相色谱法测得左旋紫草素的含量为0.01～0.96mg/g,乙酰紫草素含量为0.39～24.10mg/g,去氧紫草素含量为0.77～31.10mg/g,β,β＇-二甲基丙烯酰紫草素含量为0.25～13.20mg/g。结论新疆市场流通的新疆紫草药材羟基萘醌总色素及各成分含量差异很大。

Objective To investigate the quality of Arnebia euchroma（Royle） Johnst circulated in parts of Xingjian medicinal materials markets.Methods Ten batches of crude materials of Arnebia euchroma（Royle）Johnst in different regions of Xinjiang were collected for the total contents measurement of hydroxynaphthoquinones by UV spectrophotometry and contents measurement of four major naphthoquinones： shikonin,acetylshikonin,deoxy shikonin and β,β＇-dimethylacryl shikonin by Re-HPLC simultaneously with the chromatographic conditions,in which the Scienhome Kromasil C18column（4.6 mm × 200 mm,5μm）,acetonitrile-0.3% phosphoric acid were used as mobile phase by gradient eluting,at 35℃ with the flow rate of 1.0mL/min for 50 min,detecting at 274 nm.Results The total contents of hydroxynaphthoquinones in different samples were 0.6-17.3 mg/g by UV spectrophotometry,the content of shikonin,acetylshikonin,deoxy shikonin and β,β＇-dimethylacryl shikonin were0.01-0.96 mg/g,0.39-24.1 mg/g,0.77-31.1 mg/g and 0.25-13.2 mg/g,respectively.Conclusion There were great differences in contents of total hydroxyl naphthoquinone and four major naphthoquinones in Arnebia euchroma（Royle） Johnst circulated in different parts of Xinjiang.