津力达颗粒对1型糖尿病大鼠生长激素-胰岛素样生长因子-1轴的作用

Effects of Jinlida granules on growth hormone/insulin-like growth factor-1axis in type 1diabetic rats

目的:探讨津力达颗粒对1型糖尿病大鼠生长激素-胰岛素样生长因子-1(GH/IGF-1)轴的作用。方法:采用链脲佐菌素(STZ)60mg/kg腹腔注射制备1型糖尿病SD大鼠模型,随机分为7组:糖尿病模型组、低/中/高剂量津力达组、中剂量津力达+通心络组、二甲双胍组、沙格列汀组,另设正常对照组,每组5只。分别予以药物或安慰剂灌胃给药,8周后测定空腹血糖(FBG)、糖化血红蛋白(HbA1C)、空腹胰岛素(FINS)、C肽、生长激素(GH)、胰岛素样生长因子-1(IGF-1)、胰岛素样生长因子结合蛋白-1(IGFBP-1);实时(real-time)测定肝脏生长激素受体(GHR)mRNA。结果:与模型组相比,中、高剂量津力达组的FBG、HbA1C显著降低(P<0.05,P<0.01),FINS、C肽显著升高(P<0.05,P<0.01);各药物治疗组血清GH显著降低(P<0.01),IGF-1显著升高(P<0.01)。结论:津力达颗粒可改善GH/IGF-1轴紊乱。

Objective：To investigate the effect of Jinlida granules on the regulation of growth hormone/insulin-like growth factor-1（GH/IGF-1）axis in type 1diabetic rats.Methods：Type 1diabetic SD rats were induced by intraperitoneal injection of streptozotocin（STZ）60mg/kg.The diabetic rats were randomly divided into 7groups：the diabetic model group,the low/median/high doses of Jinlida granules groups,the median Jinlida ＋ Tongxinluo group,the metformin group and the saxagliptin group,each consisting of 5rats.Another 5normal rats were assigned as control.The rats were administered drugs or placebo by gavage,respectively.After 8weeks,diabetic parameters were detected,including fasting blood glucose（FBG）,glycosylated hemoglobin Alc（HbA1C）,fasting insulin（FINS）,C peptide,growth hormone（GH）,IGF-1,insulin-like growth factor binding protein-1（IGFBP-1）.Hepatic growth hormone receptor（GHR）mRNA was detected by real-time PCR.Results：Compared with the levels of the diabetic model group,the levels of FBG and HbA1 C were significantly decreased（P〈0.05,P〈0.01）in median and high dose Jinlida groups,and the levels of FINS and C peptide were significantly increased（P〈0.05,P〈0.01）.Decreased serum GH levels were also detected in the median and high dose Jinlida groups,as compared with that of the diabetic model group（P〈0.01）,and IGF-1level was increased（P〈0.01）.Conclusion：Jinlida granules could improve the disorder of GH/IGF-1axis in diabetic rats.