脾源性酪氨酸激酶基因在奶牛乳腺中的表达研究

Study on Expression of SYK in Dairy Cow Mammary Gland

为探讨脾源性酪氨酸激酶(spleen tyrosine kinase,SYK)的表达与奶牛乳腺发育和泌乳功能之间的关系,试验采用Western blotting和激光共聚焦显微技术对泌乳期高乳品质、低乳品质及干乳期的中国荷斯坦奶牛乳腺组织中SYK的表达含量和表达部位的变化进行研究。结果表明,干乳期奶牛乳腺组织中SYK的表达显著高于泌乳期奶牛乳腺组织(P<0.05),泌乳期高乳品质、低乳品质奶牛乳腺组织中SYK的表达差异不显著(P>0.05);在干乳期SYK主要在乳腺导管上皮细胞的胞质中表达,而在泌乳期SYK在腺泡上皮细胞中表达。结果提示SYK是乳腺上皮细胞增殖与分化的调节因子,主要参与干乳期乳腺组织的重建过程。

To investigate the relationship between the expression of SYK and dairy cow mammary gland development and lactation, the expression of SYK in lactating dairy cow mammary gland with high or low quality milk and dry period Holstein dairy cow mammary gland was detected by Western blotting and laser confocal microscope. The results showed that SYK expression in dry period mammary gland was significant higher than that in lactating mammary gland （P〈0.05）. There was no SYK differential expression detected between lactating mammary gland with high quality milk and low quality milk （P〉0.05）. SYK was mainly located in the cytoplasm of ductal epithelial cells in dry period mammary gland. In lactating mammary gland, SYK was existed in aci- nar epithelial cells. All these results revealed that SYK was a regulator in mammary epithelial cell proliferation and differentiation. It participated in mammary gland reconstitution in dry period.