摘要
利用ISSR分子标记对雄性猕猴桃16个材料进行遗传多样性分析。从100条引物中筛选出10条引物用于ISSR扩增,共扩增出172条带,其中多态性条带140条,多态性百分率为81.4%;经POPGENE 1.32软件分析结果显示,16个雄性猕猴桃材料的遗传距离在0.1503-0.5128之间,平均Nei＇s基因多样性指数（H）为0.2416,平均Shannon信息指数（I）为0.4048;聚类分析结果显示,在遗传相似系数为0.64处可将供试材料分成4类,第Ⅰ类为中华和美味猕猴桃,第Ⅱ类为阔叶、毛花猕猴桃,第Ⅲ类为魁绿猕猴桃,第Ⅳ类为四萼猕猴桃。结果表明ISSR可用于雄性猕猴桃遗传多样性研究,该研究结果可为猕猴桃种质资源的进一步开发利用提供重要信息。
In this study,16 male kiwifruit cultivars were analyzed using inter-simple sequence repeat markers（ ISSR） to investigate the genetic diversity. 10 primers selected from 100 primers were used for ISSR amplification.A total of 172 bands were generated,of which 140 bands were polymorphic bands（ the percentage of polymorphic band,PPB = 81. 4%）. The results of POPGENE version 1. 32 analysis showed that the genetic distance of the 16 male kiwifruit cultivars was between 0. 1503-0. 5128 and the genetic diversity index（ H） and Shannon index（ I）was 0. 2416,0. 4048,respectively. These cultivars were divided into 4 groups at the genetic similarity of 0. 64. ClassⅠ was made up of A. chinensis and A. deliciosa. Class Ⅱ was A. latifolia and A. eriantha. Class Ⅲ was ＇Kuilv＇ and Class Ⅳ was A. tetramera. It was indicated that ISSR markers were feasible to analyze the genetic diversity of male kiwifruits and the results would facilitate the explotation and utilization of kiwifruits.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2015年第3期618-623,共6页
Journal of Plant Genetic Resources
基金
四川省国际科技合作与交流研究计划项目(2013HH0020)
2013年四川省公益性科研院所基本科研业务费项目
猕猴桃育种及利用四川省重点实验室
关键词
猕猴桃
雄株
ISSR
聚类分析
遗传多样性
kiwifmit
male cultivars
ISSR
cluster analysis
genetic diversity
