辽宁省主栽水稻品种抗稻瘟病基因的鉴定及分析

Identification and Analysis of Rice Blast Resistance Gene Alleles in 24 Rice Cultivars from Liaoning Province

鉴定主栽水稻品种及育种骨干亲本的抗瘟基因型,有助于了解不同抗病基因在品种中的分布,为抗病品种选育及品种布局提供参考。本研究选取辽宁省24份水稻材料,根据7个抗稻瘟病基因的保守区设计引物,扩增各品种的编码区序列,对扩增的序列进行对比分析,鉴定各基因在24个品种中的分布情况。结果表明:辽宁省24个主栽水稻品种均不携带Pi21、Pi36、Pi37或其抗病等位基因,而Pid2、Pid3、Pita和Pik/Piks/Pikm/Pikp在24个品种中以不同突变类型及不同频率出现,其中,在2个品种中检测到Pid2抗病基因及Pid3的抗病等位基因;4个品种检测到Pita的抗病等位基因,Pita的等位基因中新发现的几处碱基突变并未影响抗病基因的功能;所有品种中均无与Pik及其复等位基因完全一致的序列,但利用携带AVR-pik的稻瘟病菌接种鉴定结果表明,辽粳454和沈农265携带的Pik等位基因可能仍具有抗病基因功能。

Identification on rice blast resistance genes in main rice cultivars would contribute to understand the distribution of resistance genes, which can guide rice disease resistant varieties breeding and cultivating. In this study,24 cultivars from Liaoning province were used to study the distribution of resistance genes and the function of their alleles. Primers were designed according to the conservative region sequence of nine genes to amplify the coding region of 24 cultivars. The amplification results from 24 cultivars were compared, which suggested that the 24 cuhivars from Liaoning province did not carry the Pi21 ,Pi36,Pi37 and their resistant alleles. However, Pid2 ,Pid3, Pita and Piks/Piks/Pikm/Pikp existed in the 24 varieties with different genotypes and mutation frequencies. Among these resistance genes, the resistance alleles of Pid2 and Pid3 were detected in 24 cultivars. The alleles of Pita were detected in four cultivars and the new mutations did not change the founction of the Pita. The same sequences of Piks/Piks/Pikm/Pikp were not found in the 24 cuhivars. After being inoculated with rice blast fungus carried AVRPik, Liaojing454 and Shennong265 were all resistant to the pathogens, which showed that the alleles carried by Liaojing454 and Shennong265 might serve resistance functions.