结肠癌组织IL-10和IFN-γ mRNA的表达及其临床意义

Expression and Clinical Significance of IL-10 and IFN-γ mRNA in Colon Cancer Tissue

目的:研究结肠癌组织白细胞介素-10(IL-10)和干扰素-γ(IFN-γ)的基因表达及其相关的启动子区甲基化状态。方法:随机选取2011年1月-2014年6月80例结肠癌患者组织为结肠癌组、80例相应癌旁组织为癌旁组和100例正常健康者结肠组织为对照组。采用亚硫酸氢盐测序法、甲基化特异性PCR和实时荧光定量PCR检测对IL-10和IFN-γ基因的甲基化状况进行检测,分析其与结肠癌主要临床特征之间的关系。结果:三组组织的IL-10和IFN-γm RNA水平之间比较,差异均有统计学意义(P<0.05),其中结肠癌组和癌旁组组织的IL-10 m RNA均高于对照组(P<0.05),结肠癌组和癌旁组组织的IFN-γm RNA均低于对照组(P<0.05);三组IL-10基因启动子区-110,-185位点甲基化率比较,差异均有有统计学意义(P<0.05),其中结肠癌组和癌旁组组织的IL-10基因启动子区-110均高于对照组(P<0.05),结肠癌组组织的IL-10基因启动子区-185低于对照组(P<0.05);IFN-γ基因启动子区甲基化率结肠癌组>癌旁组>对照组,差异均有统计学意义(P<0.05)。结论:IL-10和IFN-γ基因启动子区甲基化状态影响其在结肠癌组织中的表达,结肠癌患者处于TH2极化状态,与结肠癌发生发展密切相关。

Objective：To investigate the gene expression of interleukin-10（IL-10） and interferon-γ（IFN-γ） colon tissue and methylation status of the promoter region.Method：Randomly selected from January 2011 to June 2014 colon cancer patient’s tissue 80 cases of colon cancer group, 80 cases of corresponding adjacent tissues as adjacent group, ad 100 cases of normal healthy colon tissue as the control group.Using bisulfite sequencing method （BSP）,methylation specific PCR（MSP） and real-time quantitative PCR （RT-PCR） detection of promoter methylation of three groups of patients with colonic tissue IL-10 and IFN-γ gene and mRNA expression status.Result：Comparison of the three groups in the tissue IL-10 and IFN-γ mRNA, the differences were statistically significant（P〈0.05）.Colon cancer group and adjacent group of IL-10 mRNA were higher than the control group（P〈0.05）,IFN-γ mRNA were lower than in the control group（P〈0.05）.Three groups of IL-10 gene promoter region-110,-185 sites methylation rates,the difference was statistically significant（P〈0.05）.Colon cancer group and adjacent group organization of IL-10 gene promoter region -110 were higher than the control group（P〈0.05）,colon cancer group IL-10 gene promoter region-185 were lower than in the control group（P〈0.05）;IFN-γ gene promoter methylation of the colon cancer group〉the adjacent group〉the control group, the differences were statistically significant（P〈0.05）.Conclusion：IL-10 and IFN-γ gene promoter methylation status affect its expression in colon cancer, colon cancer patients in TH2 polarization state, closely related to the development of colon cancer.