毛蚶抗菌活性筛选及低极性组分GCMS分析

Antibacterial activities and GC- MS analysis of low polar components from Scapharca subcrenata Lischke

目的:研究毛蚶的抗菌活性部位及有效成分。方法:毛蚶软组织经乙醇提取后,采用液液萃取法将提取物分为石油醚相、乙酸乙酯相、正丁醇相和水相4个不同的极性部位;采用抑菌圈法和最小杀菌浓度测定法,对毛蚶乙醇提取物和各极性部位进行了抗菌活性测定,并用正相硅胶柱对抗菌活性较好的石油醚相进行了分离纯化,得到甾体组分;采用GC-MS技术对石油醚相和甾体组分进行了化学成分分析。结果:毛蚶石油醚相的抑菌效果最好;从毛蚶石油醚相中鉴定出了30个化合物,主要为脂肪类和甾醇类化合物;从甾体组分中鉴定出8个化合物,其中7个为首次从毛蚶中分离鉴定。结论:毛蚶的石油醚相具有明显的抗菌活性,石油醚相以甾醇类化合物为主。

Objective-To screen the antibacterial fractions and constituents of Scapharca subcrenata Lischke. Methods :The soft tissues of S.subcrenata was extracted by ethanol.Liquid-liquid extraction with petroleum ether, ethyl acetate,n-butanol and water was used for the separation of ethanolic extract and 4 organic fractions were obtained.The antibacterial activities of the ethanolic extract and organic fractions was evaluated by diameter of inhibition zone(DIZ) and the minimum bactericidal concentration (MBC).The active petroleum ether fraction was separated and purified with silica gel column chromatography,and a sterol mixture was obtained.The compounds in the petroleum ether fraction and the sterols were analyzed by gas chromatography-mass spectrometry(GC- MS).Results:The antibacterial substances were mainly in the petroleum ether fraction, and 30 compounds were identified by GC-MS, most of which were I ipids and sterols.Eight compounds were identified by GC-MS from the sterol component, and seven compounds were identified for the first time from S. subcrenata. Conclusion, The antibacterial activities of petroleum ether fraction from S. subcrenata were obvious, and sterol was the major compound of petroleum ether fraction.