应用响应面法优化叶黄素降解发酵培养基

Optimization of fermentation medium for lutein degradation applying response surface methodology

利用响应面分析法对Pantoea dispersa(Y08)菌降解叶黄素产香的培养基进行了优化。采用Box-Behnken实验设计,选定KH2PO4、蔗糖和混合氮源(酵母膏∶天门冬酰胺=2∶1)3个关键因子为响应因子,以叶黄素降解率为响应值建立多元二次回归方程,在分析各个因素的显著性和交互作用后,确定了Pantoea dispersa菌降解叶黄素的最优培养基为:蔗糖0.97%,混合氮源(酵母膏∶天门冬酰胺=2∶1)1.38%,KH2PO40.15%,叶黄素降解率为80.03%,与理论预测值基本吻合,比优化前提高140.67%。

Optimization of fermentation medium using for lutein degradation to form important aroma compounds with Pantoea dispersa Y08 strain was performed employing the response surface analysis(RSA) method.Based on single factor experiment,three critical factors (the content of sucrose, mixed nitrogen sources and KH2 POt )were selected as response factors.The quadric regression equation was established according to the lutein degradation yield.As a result, the optimum fermentation medium was composed of sucrose 0.97%, mixed nitrogen sources (yeast extract'asparagine =2:1 )1.38% and KH2PO4 0A5%.Under the situation,the highest yield of degradation of lutein was predicted to be 80.03%.After optimization,the lutein degradation yield was increased by 140.67%.The experimental values were in according with the predicted values.