绿原酸对高脂饲喂SD大鼠脂肪组织三酰甘油合成降解关键酶基因表达的影响

Study on the effect of chlorogenic acid on the expression of key enzyme genes involved to triglyceride synthesis and degradation in high- fat diet- fed Sprague- Dawley rat adipose tissue

目的:研究绿原酸对高脂饲喂SD大鼠附睾脂肪组织三酰甘油合成及代谢关键酶基因表达的影响,探讨绿原酸改善肥胖的新靶点。方法:雄性SD大鼠随机分成对照组(NC)、高脂饲料模型组(HFD)、高脂饲料+绿原酸低、高剂量组(20、90mg·kg-1·d-1,HFD-LC、HFD-HC)和罗格列酮组(ROS),饲养12周后处死,测定体脂含量变化以及附睾脂肪组织中二酰甘油酰基转移酶(Diacylgycerol acyltransferase 2,DGAT2)、甘油三脂脂肪酶(Adipose triglyceride lipase,ATGL)和激素敏感脂肪酶(Hormone-sensitive lipase,HSL)mRNA表达水平。结果:与NC组相比,HFD组体脂含量、DGAT2、HSL mRNA表达水平明显升高,ATGL mRNA表达水平下降不显著。与HFD组相比,HFD-LC、HFD-HC对体脂率、DGAT2 mRNA影响不明显(p>0.05),但能极显著提高ATGL mRNA表达水平,HFD-HC组HSL表达水平也极显著上升。结论:绿原酸能显著提高大鼠附睾脂肪组织ATGL和HSL mRNA表达水平,有效控制体脂堆积。

Objective:In order to explored the effect of chlorogenic acid on the expression of key enzyme genes involved to triglyceride synthesis and degradation in high-fat diet-fed SD rat adipose tissue. Methods:Male SD rats were randomly divided into 5 groups:normal control group (NC), high-fat diet group (HFD), HFD with low- dose CG( 20mg/kg ; HFD- LC) group, HFD with high- dose CGA ( 90mg/kg ; HFD- HC) group and HFD with rosiglitazone group( ROS).All mice were killed after 12 weeks and body fat mass were detected. Expression of mRNA of DGAT2,ATGL and HSL were assessed by real time PCR.Results:The body fat content, level of DGAT2, HSL mRNA of HFD rats were higher than those of NC rats,while there was no significant difference between NC and HFD rats in the expression of ATGL, The level of ATGL mRNA in HFD-LC and HFD-HC rats were higher than those of HFD rats, HSL mRNA expression in HFD- HC was significantly higher than those in HFD, but it had no significant difference between HFD and HFD-LC, HFD-HC in the expression of DGAT2 mRNA and the body fat mass.Conclusion:Chlorogenic acid enhanced ATGL and HSL mRNA expression in epididymis adipose tissue of SD rat.