超快速液相色谱-串联质谱法测定水果中多菌灵等7种农药残留量

Determination of 7 Pesticide Residues in Fruits by Altra Fast Liquid Chromatography-tandem Mass Spectrometry

本文采用超快速液相色谱一串联质谱法，建立了水果中7种多残留农药的快速检测方法。水果样品经乙腈溶液提取、盐析后吸取上清液，以甲醇-0．1％甲酸水溶液作为流动相体系，在电喷雾正离子模式下，进行多反应监测模式测定。结果表明：7种农药标样的响应值与质量浓度在1．0～200μg／L范围内均呈良好的线性关系，相关系数均达到0．99，各农药的检出限（LOD）范围为0．05～0．20μg／kg，方法定量限（LOQ）范围为0．10～0．60μg／kg。7种农药的平均回收率（n=6）范围为82％～114％．相对标准偏差（RSD）小于10％。该方法快速、高效，能够满足高原地区水果中7种多残留农药的快速分析检测要求。

This article used the ultra fast liquid chromatography-tandem mass spectrometry, estab- lished a rapid detection of seven pesticide residues in fruits. The fruit samples were extracted by acetoni- trile solution, after salting out from the supernatant, used methanol and 0. 1% formic acid aqueous solu- tion as mobile phase system, used the mode of positive ions electrospray, the determination of multiplereaction monitoring mode. The results showed that： all the pesticides had the good linearity in the concen- tration of 1.0-200μg/L range, the correlation coefficient were higher than 0.99.The pesticide detection limit （LOD） range from 0.05 to 0.20μg/kg, the methods quantitative limit （LOQ） range from 0.10 to 0. 60μg/kg. The average recovery of 7 kinds of pesticide （n = 6） was in the range of 82% - 114%, the relative standard deviations （RSD） was less than 10%.This method was rapid, efficient, could be satis- fied to the rapid detection and analysis of 7 pesticide residues in fruits in plateau section.