摘要
本文采用超快速液相色谱一串联质谱法,建立了水果中7种多残留农药的快速检测方法。水果样品经乙腈溶液提取、盐析后吸取上清液,以甲醇-0.1%甲酸水溶液作为流动相体系,在电喷雾正离子模式下,进行多反应监测模式测定。结果表明:7种农药标样的响应值与质量浓度在1.0~200μg/L范围内均呈良好的线性关系,相关系数均达到0.99,各农药的检出限(LOD)范围为0.05~0.20μg/kg,方法定量限(LOQ)范围为0.10~0.60μg/kg。7种农药的平均回收率(n=6)范围为82%~114%.相对标准偏差(RSD)小于10%。该方法快速、高效,能够满足高原地区水果中7种多残留农药的快速分析检测要求。
This article used the ultra fast liquid chromatography-tandem mass spectrometry, estab- lished a rapid detection of seven pesticide residues in fruits. The fruit samples were extracted by acetoni- trile solution, after salting out from the supernatant, used methanol and 0. 1% formic acid aqueous solu- tion as mobile phase system, used the mode of positive ions electrospray, the determination of multiplereaction monitoring mode. The results showed that: all the pesticides had the good linearity in the concen- tration of 1.0-200μg/L range, the correlation coefficient were higher than 0.99.The pesticide detection limit (LOD) range from 0.05 to 0.20μg/kg, the methods quantitative limit (LOQ) range from 0.10 to 0. 60μg/kg. The average recovery of 7 kinds of pesticide (n = 6) was in the range of 82% - 114%, the relative standard deviations (RSD) was less than 10%.This method was rapid, efficient, could be satis- fied to the rapid detection and analysis of 7 pesticide residues in fruits in plateau section.
出处
《西藏农业科技》
2014年第4期37-41,共5页
Tibet Journal of Agricultural Sciences
关键词
水果
超快速液相色谱-串联质谱
农药残留
Fruits
Ultra fast liquid chromatography-tandem mass spectrometry
Pesticide residues