缩宫素受体在子宫腺肌症肌层平滑肌组织中的表达及与痛经的关系

Expression of oxytocin receptor in smooth muscle of myometrium and its correlation with dysmenorrhea in adenomyosis

目的探讨子宫腺肌症(adenomyosis,AM)患者子宫肌层平滑肌组织中缩宫素受体(oxytocin receptor,OTR)的表达水平与痛经程度的关联性。方法收集2013年9月-2014年8月在本院妇科因AM行全子宫切除手术的腺肌症患者50例为腺肌症组,同期因子宫肌瘤行全子宫切除的患者20例为对照组。应用免疫组化染色法(S-P法)检测20例正常子宫肌层、50例子宫腺肌症肌层平滑肌组织中的OTR表达情况。采用光学显微镜及Image Plus Pro 6.0图像分析系统测定OTR水平,并对其差异进行分析。结果 AM组子宫肌层平滑肌组织中OTR的表达高于对照组(62 370±34 758 vs 16 082±11 125,P<0.01),且表达强度随着痛经程度的加重而增加,差异均有统计学意义。结论 OTR在AM患者子宫肌层平滑肌组织中的表达与痛经程度呈正相关,推测患者痛经形式可能为宫缩痛,表明宫缩强度的增加参与了AM疼痛的发病机制。

Objective To evaluate the expression level of oxytocin receptor （OTR） in smooth muscle of myometrium and its correlation with the degree of dysmenorrhea in adenomyosis.Methods Fifty AM cases and 20 uterine myoma cases admitted to department of gynecology in our hospital from September 2013 to August 2014 were enrolled in this study. Immunohistochemical S-P method was used to identify the expression of oxytocin receptor in smooth muscle of myometrium from 20 cases with pathologically confirmed normal myometrium and 50 female patients with pathologically confirmed adenomyosis. The expression level was detected by using digital image analysis with optical microscope and Image Pro-Plus 6.0. ResultsThe positive expression rates of OTR in smooth muscle of myometrium in AM group were higher than that in controls （62 370±34 758vs 16 082±11 125, P〈0.01）, and the expression level increased with the aggravation of dysmenorrhea. All the differences were statistically significant. ConclusionThe expression level of OTR in smooth muscle of myometrium is positively correlated with the degree of dysmenorrhea. This indicates that the increased intensity of uterine contraction is involved in the pathogenesis of dysmenorrhea.