禽源空肠弯曲杆菌ERIC-PCR分型及毒力因子研究

Study on ERIC-PCR Typing and Virulence Genes of Avian Campylobacter jejuni

[目的 ]通过对禽源空肠弯曲杆菌分子分型和毒力研究,了解禽源空肠弯曲杆菌在禽肉产品生产过程中的主要传播途径和携带的主要致病因子。[方法 ]对从家禽养殖场、屠宰场等采集的720份泄殖腔拭子和禽肉表面拭子样品分离的311株空肠弯曲杆菌进行ERIC-PCR分型和16种毒力因子检测。[结果 ]禽源空肠弯曲杆菌的分离率为43.19%;肉鸡生产链中空肠弯曲杆菌沿生产链传播,且有优势菌株型;90%以上分离菌株携带11种毒力基因。[结论 ]肉鸡生产链中空肠弯曲杆菌存在水平传播现象,且没有外源性污染;16种毒力基因中,14种毒力基因的携带率均在50%以上。加强禽源空肠弯曲菌的监测是控制空肠弯曲菌的前提,对公共卫生具有重要意义。

To understand the main transmission route and pathogenic factors of Campylobacterjejuni （C.jejuni） during poultry production process, 720 samples were collected from poultry cloaca and chicken meat for isolation and identification of C.jejuni resulting in 311 C..jejuni isolates. ERIC-PCR typing and virulence of the 311 C. jejuni isolates were analyzed with PCR assay. The result revealed that more than 90% isolates carried 11 virulence genes. C..jejuni was spread along the broiler production chain, and there existed dominant strains. In conclusion C. jejuni was horizontally transmitted in broiler production chain without exogenous contamination. 50% or more C. jejuni isolates carried 14 virulence genes. It is significant for public health to strengthen the monitoring and control of C. jefuni from avian.