甲状腺组织中免疫调节T淋巴细胞的比例

Proportion of Immune Regulatory T Cells in Thyroid

目的观察正常甲状腺及Graves病(GD)患者甲状腺组织中免疫调节T淋巴细胞的比例。方法采用流式细胞术分别检测20例正常甲状腺及9例GD患者甲状腺中Treg细胞、Th1细胞、Th2细胞比例,并与外周血进行对比。结果外周血、正常甲状腺、GD甲状腺中Treg细胞比例分别为1.79±0.43%、17.07±10.16%、20.87±13.26%,Th1细胞比例分别为14.45±2.46%、17.28±3.02%、8.62±1.88%,Th2细胞比例分别为1.44±0.31%、1.78±0.55%、6.87±3.27%。与外周血相比,正常甲状腺及GD甲状腺中Treg细胞比例明显增高(P<0.01),与正常甲状腺相比,GD甲状腺中Th1细胞比例明显降低(P<0.05),Th2细胞比例明显升高(P<0.01)。结论甲状腺组织局部存在着数量明显增多的Treg细胞,GD患者的甲状腺中存在Th1/Th2细胞失衡。

Objective To evaluate the proportion of immune regulatory T cells in normal thyroid and GD thyroid. Methods The proportion of regulatory T ceils （Tregs）, T helper t （ThI） ceils, Th2 ceUs in 20 normal thyroids and 9 thyroids of GD patients were analyzed by flow cytometry and then compared to the proportion in peripheral blood. Results In peripheral blood, normal thyroid and GD thyroid, the proportion of Tregs were 1.79 ± 0.43%, 17.07 ± 10.16%, 20.87 ± 13.26% ; the proportion of Thl cells were 14.45 ± 2.46%, 17.28 ± 3.02% , 8.62 ± 1.88% , and the proportion of Th2 cells were 1.44 ±0.31% , 1.78 ±0.55% , 6.87 ± 3.27%. Compared to peripheral blood, the proportion of Tregs was significantly increased in normal thyroid and GD thyroid （P 〈 0.01 ）. Compared to normal thyroid, the proportion of Thl cells significantly decreased （P 〈0.05） and the proportion of Thl cells significantly increased in GD thyroid （P 〈0.01 ）. Conclusion Tregs are abundant in thyroid tissue and there is an imbalance of Thl/Th2 cells in GD thyroid tissue.