人乳头瘤病毒E6E7原癌蛋白在客家地区非小细胞肺癌中表达的意义

Significance of the expression of human papilloma virus proto-oncogene proteins E6E7 in non-smallcell lung cancer in Hakka region

目的探讨人乳头瘤病毒E6E7原癌蛋白在客家地区非小细胞肺癌（NSCLC）中表达的意义。方法应用免疫组化（SABC法）检测43例客家地区NSCLC患者及27例肺良性病变患者中HPVE6、E7原癌蛋白的表达水平。采用SPSS16．0软件包进行统计学处理，HPVE6、E7原癌蛋白在正常肺组织和NSCLC组织中表达，以及在NSCLC患者不同临床及病理特征中表达的比较，采用卡方检验或四格表精确概率法。结果NSCLC中HPVE6、E7原癌蛋白的表达总阳性率为39．5％（17／43），而肺良性病变组阳性率为7．4％（2／27），差异有统计学意义（z。=8．657，P〈O．05）；HPV感染在吸烟NSCLC患者中阳性率高达53．8％，而不吸烟NSCLC患者中阳性率仅为19．0％，两者差异有统计学意义（χ2=5．79，P〈0．05）；不同组织学类型（腺癌中阳性率为43．5％，鳞癌中阳性率为35．0％，χ2=0．322，p〉O．05）、年龄（〈50岁阳性率为33．3％，≥50岁阳性率为40．5％，校正X。=0．000，P〉O．05）及性别（男性阳性率为40．0％，女性阳性率为30．8％，校正χ2=0．054，P〉0．05）HPV感染阳性率差异无统计学意义。结论HPVE6、E7原癌蛋白的表达在NSCLC发生中可能有病因学意义，吸烟者HPV感染率较高，而不同NSCLC的组织学类型和患者不同性别的HPV感染率差异无显著意义。

Objective To investigate the clinical and pathological features of non-small-cell lung cancer（NSCLC） infected by human papillomavirus in Hakka region.Methods SABC immunohistoehemical method was used to detect the expression of HPV E6, E7 in forty-three cases with NSCLC and in twenty- seven benign lung specimens, respectively.The expression of the HPV E6, E7 in NSCLC and benign patients was analyzed by Fisher＇s exact test and chi-square test. Results The overall positive rate of HPV E6, E7 expression in NSCLC was 39.5% （17/43） ,which had a significant difference compared with the data of the benign group（7.4% ,2/24）（ χ2= 8.657, P 〈0.05）.Among all the NSCLC patients,the HPV positive rate of smokers（53.8%） was statistically higher than that of nonsmokers （19.0%）, which showed a positive correlation between HPV infection and cigarettes（ χ2= 5.79, P 〈0.05 ）.However, no significant difference had been found among HPV infection and other clinical or pathological factors such as classification of histology （positive rate of adenoearcinoma was 43.5%, and squamous carcinoma was 35.0%,χ2=0.322, P 〉0.05） , age（ under the age of 50,positive rate was 33.3% ,those older than 50 was 40.5%, correctedχ2 ~ 0.000, P 〉 0.05） or gender （ male had a positive rate of 40.0%, female 30. 8%, corrected χ2 = 0. 054, P 〉 0.05）. Conclusions HPV E6,E7 may have etiologic sense for NSCLS,the HPV positive rate of smokers is higher than that of nonsmokers,and the HPV positive rate of different histological types and sex has no significantdifference between two groups.