调节性T细胞增龄性变化与老年肺癌发病率的关系

The relationship between aged-related change of regulatory T cells and incidence of lung cancer in the elderly

目的探讨CD4^+CD25^+Foxp3^+调节性T(Treg)细胞增龄性变化与老年肺癌发病率的关系。方法建立Lewis肺癌模型36只C57BL/6鼠按月龄不同分为6组分别为青年(1月龄)、中年(6月龄)、老年(12月龄)健康组及其对应年龄肺癌组,每组各6只。提取小鼠脾脏,分离淋巴细胞采用流式细胞术测定CD4^+CD25^+Foxp3^+Treg占CD4^+T细胞的比值(CD4^+CD25^+Foxp3^+/CD4),用均数±标准差表示健康与肺癌两组间CD4^+CD25^+Foxp3^+/CD4值比较采用独立样本t检验,同一组内不同年龄的比较采用单因素方差分析。结果肺癌鼠脾脏CD4^+CD25^+Foxp3^+/CD4^+值为(7.96±3.22)显著高于健康组(2.56±0.95)差异有统计学意义(t=4.33,P<0.05):在健康组内,青年、中年、老年三组CD4^+CD25^+Foxp3^+/CD4分别为(1.35±0.32)、(2.13±0.59)、(3.58±0.74),呈增龄性递增(F=23.13,P<0.05);老年肺癌组鼠脾脏细胞中CD4^+CD25^+Foxp3^+/CD^+值为(11.97±3.62),高于青年、中年组(F=33.89,P<0.05)。结论 CD4^+CD25^+Foxp3^+Treg细胞增龄性变化与老年肺癌高发病率紧密相关。

Objective To study the change of age-related regulatory T cells and its relationship with the high incidence of lung cancer in the elderly patients. Methods To set up Lewis lung cancer model, thirty-six C57BL/6 mice were divided into six groups according to the different months of age.The change of CD4＋CD25＋ Foxp3＋ Treg cells in spleen was evaluated among the six groups by flow cytometry method. Results The number of CDg＋CD25＋Foxp3＋ Treg cells was higher in tumor group （7.96±3.22） than that in healthy group（2.56±0.95） at the same age （ CD4＋ CD25＋ Foxp3＋/CD4＋, t = 4.33, P 〈0.05 ）. Besides, in the healthy groups, there was statistical difference among the three groups （F = 23.13, P 〈0.05）.Accumulation of the C D4＋ CD25＋Foxp3＋ Treg cells were accompanied with age, the elderly mice contained a significantly larger population of CD4＋CD25＋Foxp3＋ Treg cells in their spleen than those in younger counterparts, and the elderly tumor group had the highest expression （11.97 ± 3.62 ）. Conclusion The results suggest a close relationship between the age-related changes of treg ceils aging and the incidence of lung cancer in the elderly.