后肢振动对雄性新西兰兔精浆脂质过氧化指标及精子DNA的影响

Effects of hind legs vibration on lipid peroxidation and DNA in spermatozoa of New Zealand rabbits

目的探讨后肢振动对雄性新西兰兔精浆脂质过氧化指标及精子DNA的影响。方法将新西兰兔采用完全随机分组法分为1个对照组和3个实验组[A组(接振强度为3.02 m/s^2)、B组(接振强度为6.13 m/s^2)、C组(接振强度为12.26 m/s^2)]。实验组接振30 d后采用化学发光法测定精浆活性氧(ROS)水平,黄嘌呤氧化酶法测定超氧化物歧化酶(SOD)活性;采用原位末端转移酶标记法(TUNEL)和彗星实验检测精子DNA的损伤。结果实验组实验后精浆ROS水平高于对照组[对照组:(31.83±3.34)RLU/s,A组:(34.43±2.56)RLU/s,B组:(51.37±3.86)RLU/s,C组:(59.74±5.64)RLU/s,F=134.42,P<0.05]、SOD活性明显低于对照组[对照组:(58.45±4.29)n U/m L,A组:(51.03±3.81)n U/m L,B组:(34.60±2.39)n U/m L,C组:(32.93±3.87)n U/m L,F=139.60,P<0.05)];实验组DNA断裂的精子百分率明显高于对照组[TUNEL法:A组:(7.25±1.42)%,B组:(10.33±1.37)%,C组:(14.75±2.30)%,F=56.86,P<0.05;慧星实验:A组:(7.08±1.39)%,B组:(10.58±1.08)%,C组:(15.17±1.90)%,F=90.57,P<0.05]。结论振动通过增加精浆ROS水平,降低SOD活性,损伤精子DNA,使精子裂解,从而影响生殖功能。

Objective To study the effects of vibrations on levels of lipid peroxidation and DNA in spermatozoa and the diagnostic significance of vibrations disease.Methods Male adult rabbits were randomly assigned to control group and three vibration groups, after exposed to vibration for 30 days, semen ROS and SOD were detected by chemiluminescence method and xanthine oxidase method,TUNEL and comet assay test were used to detect the damage of sperm DNA. Results Compared with control group, after exposed to vibration for 30 days,the levels of semen ROS were much higher[ （A group：（34.43±2.56） RLU/s, B group： （51.37±3.86） RLU/s,C group： （59.74±5.64）RLU/s, F = 134.42, P 〈0.05] ,levels of semen SOD were much lower [ A group ： （ 51.03 ±3.81 ） n U/ml, B group ： （ 34.60±2.39 ） nU/ml, C group ： （ 32.93 ± 3.87） nU/ml, F = 139.60, P 〈0.05] ,the percentage of spermatozoa with damaged DNA were significant higher [ TUNEL：A group：（7.25±1.42）%,B group： （10.33±1.37）%,C group： （4.75±2.30）%, F =56.86, P 〈0.05;comet assay test：A group： （7.08±1.39）% ,B group： （10.58±1.08） % ,C group： （15.17±1.90） %, F =90.57, P 〈 0.05 ]. Conclusion The vibration may through improve ROS levels and decrease SOD levels to damage spermatozoa DNA, thereby affecting the male reproduction.