WIF-1基因甲基化及Wnt-5a蛋白在软骨肉瘤病人中表达的临床意义

THE CLINICAL SIGNIFICANCE OF WIF-1 GENE METHYLATION AND EXPRESSION OF WNT-5A PROTEIN IN PATIENTS WITH CHONDROSARCOMA

目的:研究WIF-1基因甲基化及Wnt-5a蛋白在软骨肉瘤病人中的表达情况,探讨二者在软骨肉瘤病人中的临床意义。方法:选取我院病理科2008-06～2012-06收治的软骨肉瘤病人43例,另选取软骨瘤病人43例作为对照。收集病人的病历资料及临床病理表现,通过甲基化特异性PCR检测法及免疫组织化学方法,监测两组病人WIF-1基因和Wnt-5a蛋白表达情况。结果:软骨肉瘤WIF-1基因甲基化阳性率高于软骨瘤,比较差异具有统计学意义(χ2=5.658,#P=0.003);软骨肉瘤Wnt-5a蛋白表达阳性率高于软骨瘤,比较差异具有统计学意义(χ2=4.656,*P=0.006)。黏液型软骨肉瘤Wnt-5a蛋白表达阳性率明显高于普通型软骨肉瘤,差异具有统计学意义(χ2=4.941,#P=0.002)。发生浸润转移的软骨肉瘤病人Wnt-5a蛋白表达阳性率高于未发生浸润转移的病人(χ2=4.681,P=0.003)。结论:WIF-l基因的甲基化可能导致Wif-l蛋白表达下降,在软骨肉瘤早期作用;Wnt-5a蛋白表达可能使肿瘤更具有侵袭性,二者可能存在协同作用。

Objective：To study the WIF-1 gene methylation and Wnt-5 a protein expression in patients with chondrosarcoma, and to explore the clinical significance in both patients with chondrosarcoma. Methods：Pathology our hospital from June 2008 to June 2012 were treated 43 patients with chondrosarcoma, chondroma select another 43 patients served as controls. Collection of medical records and clinical pathological manifestations,by methylation-specific PCR assays and immunohisto-chemical methods to monitor two groups of patients WIF-1 gene and protein expression of Wnt-5a. Results：Chondrosarcoma WIF-1 gene methylation was higher in cartilage tumors, the difference was statistically significant （χ^2 =5. 658, P=0. 003 ）;chondrosarcoma Wnt-5a protein expression was higher in cartilage tumors,and the difference statistically significant（χ^2=4. 656,＊P=0. 006）. Mucus type chondrosarcoma Wnt - 5 a protein expression was significantly higher than ordinary chondrosarcoma,a statistically significant difference（χ^2=4. 941,P=0. 002）. Invasion and metastasis in patients with chondrosarcoma of Wnt-5 a protein expression was higher in patients with invasion and metastasis（χ^2=4. 681,P=0. 003） did not occur. Conclusions：WIF-l gene methylation may lead to Wif-l protein was decreased,early role in chondrosarcoma;protein Wnt-5a may make tumors more ag-gressive,there may be a synergistic effect between the two.