MMP-11在胃癌组织的表达及临床意义

Expression of MMP-11 in Gastric Cancer Tissues and Its Clinical Significance

目的观察MMP-11蛋白在胃癌组织的表达。方法采用免疫组织化学技术检测436份胃癌组织及92份邻近正常胃黏膜组织石蜡标本MMP-11表达情况。结果 MMP-11在正常胃黏膜组织的表达低于胃癌组织(0 vs 47.5%,x2=73.424,P<0.01)。MMP-11表达与胃癌患者肿瘤大小、分化、侵犯深度、分期、淋巴转移、远处转移、脉管侵犯、Lauren分型相关(P<0.05),而与组织类型、性别无关。肿瘤逸5cm者MMP-11阳性表达率62.8%,明显高于肿瘤<5cm者的36.7%(x2=28.781,P<0.001);弥漫型者MMP-11阳性表达率明显高于肠型者(85.9%比10.7%,x2=246.751,P<0.001);T3、T4期者MMP-11阳性表达率分别为58.6%、96.2%,明显高于T1、T2期的10.5%、30.3%(x2=80.969,P<0.001);芋期、郁期胃癌患者MMP-11阳性表达率分别为68.8%、95.7%,高于玉期、域期患者的7.8%、14.4%(x2=198.170,P<0.001);脉管侵犯者MMP-11阳性表达率明显高于无脉管侵犯者(70.0%比16.4%,x2=122.193,P<0.001);淋巴结转移胃癌患者的MMP-11阳性表达率为68.9%,高于无淋巴结转移的12.7%(x2=130.382,P<0.001);有远处转移者的MMP-11阳性表达率95.1%,高于无远处转移者的39.7%(x2=64.455,P<0.001)。根据多因素Logistic回归分析,MMP11的表达在TNM分期高、脉管侵犯、有远处转移的患者中发生率高。结论 MMP-11或参与了胃癌的浸润转移进程,检测MMP-11的表达可作为判断胃癌生物学行为的重要参考指标。

Objective To investigate the expression of MMP-11 in gastric cancer. Methods MMP-11 expression in sample tissues from 436 cases with gastric cancer and 92 cases of cancer adjacent gastric mucosa was detected immunohisochemically. Results MMP-11 expression in cancer adjacent gastric mucosa was significantly lower than that in gastric cancer tissues （0 vs 47.5% χ^2=73.424, P〈0.01）. MMP-11 expression was related to tumor size, differentiation, depth of invasion, stage, lymph node metastasis, distant metastasis, vascular invasion, and Lauren classification of gastric cancer（P〈0.05）, but was not associated with histologic type and gender of patients（P〉0.05）. MMP-11 positive rate in gastric cancer patients with tumor size ≥5cm was significantly higher than that in patients with tumor size 〈5cm （62.8% vs 36.7%; χ^2=28.781, P〈0.001）; that in patients with diffusing type cancer increased to 85.0% as compared with 10.7% in patients with intestinal-type cancer （χ^2=246.751, P〈0.001）; that in patients at stage T3 and T4 was 58.6% and 96.2%, respectively, which were higher than that in patients at stage T1 and T2（10.5% and 30.3%, χ^2=80.969, P〈0.001）; that in patients with stage Ⅲ, Ⅳ cancer（68.8%, 95.7%）were significantly higher than that in patient with stage Ⅰ, II cancer （7.8%, 14.4%; χ^2=198.170, P〈0.001）; that in patients with vascular invasion was 70.0% as compared with 16.4% of patients without vascular invasion MMP-11 （χ^2=122.193, P〈0.001）; that in patients with lymph node metastasis was higher than that in patients without lymph node metastasis（68.9% vs 12.7%; χ^2=130.382, P〈0.001）; that in patients with distant metastasis increased to 95.1%as compared with 39.7% in patients without distant metastasis（χ^2=64.455, P〈0.001）. Multi-factor logistic regression analysis showed that MMP-11 high expression was seen in patients with high TNM staging, vascular invasion, or＆amp;nbsp;distant metastasis. Conclusion MMP-11 may involve in process of invasion and metastasis of gastric cancer. MMP-11 could be used as an important reference indicator for evaluation of biological behavior of gastric cancer.