鸡蛋花挥发性成分的气相特征图谱及含量测定

GC Characteristic Spectra and Determination of Volatile Components from Drying Flowers of Plumeria rubra cv. Acutifolia

目的:采用气相色谱法(gas chromatography,GC)建立鸡蛋花中挥发性成分的特征图谱,测定芳樟醇、香叶醇和橙花叔醇的含量,为该药材的质量控制提供参考。方法:采用GC测定20批鸡蛋花和3批红鸡蛋花中指标成分含量,建立鸡蛋花中挥发性成分的GC特征图谱,考察鸡蛋花和红鸡蛋花特征图谱的差异性。结果:鸡蛋花特征图谱中指定了11个共有色谱峰,3批红鸡蛋花与鸡蛋花的对照图谱明显不同,23批样品聚分为2类,其中红鸡蛋花为一类。20批鸡蛋花药材中挥发性成分的含量差异较大。结论:建立的鸡蛋花挥发性成分特征图谱特征性强,方法快速简便,能够表征鸡蛋花药材的内在信息,结合主要成分含量测定可用于鸡蛋花中挥发性成分的质量控制。

Objective： To establish characteristic spectra of volatile components in drying flowers of Plumeria rubra cv. Acutifolia by gas chromatography （GC） and determine contents of linalool, geraniol and nerolidol. Method： GC was employed to determine contents of index ingredients in drying flowers of 20 batches of P. rubra cv. Acutifolia and 3 batches of P. rubra, then GC characteristic spectra of volatile components in drying flowers of P. rubra cv. Acutifolia was established in order to investigate differences between P. rubra cv. Acutifolia and P. rubra. Result： Eleven peaks were selected as characteristic peaks to assess similarities of different samples from different origins. There were significant differences in characteristic spectra between P. rubra cv. Acutifolia and P. rubra. Samples were divided into two categories, P. rubra was one of them. Contents difference of volatile ingredients in 20 batches of P. rubra cv. Acutifolia were great. Conclusion： This method is quick and simple, which can be used as an effective mean for quality evaluation of volatile ingredientsin P. rubra cv. Acutifolia.