复方调元散挥发性成分分析

Analysis of Volatile Components of Tiaoyuan Powder

目的:研究复方调元散中的挥发性成分,探讨其治疗自闭症及相关症状的药效物质基础。方法:分别采用顶空固相微萃取法(HS-SPME)、水蒸气蒸馏法(SD)结合气质联用技术(GC-MS)对复方中挥发性成分进行分析鉴定;采用HSSPME-GC-MS法对煎煮前后复方调元散挥发性成分进行对比分析。结果:SD提取的复方挥发油中共检测出48种挥发性成分,鉴定出28种,占挥发性成分总量的85.44%;HS-SPME从煎煮前复方粉末中检测出挥发性成分122种,鉴定出73种,占挥发性成分总量的75.56%,从煎煮后复方浸膏中检测出挥发性成分50种,鉴定出27种,占挥发性成分总量的93.49%。3次不同试验共鉴定出复方挥发性成分97种,含量最高的为α-细辛脑。结论:该研究为复方调元散的挥发性成分分析提供了方法学基础,同时也为其治疗自闭症药效物质基础研究提供了参考依据。

Objective： Study the volatile components of Tiaoyuan powder and explore its pharmacodynamic material basis for the treatments of autism and related symptoms. Method： Headspace solid phase micro-extraction （HS-SPME） and steam distillation （SD） combined with gas chromatography and mass spectrometry technique （GC-MS） were used to analyze and identify volatile components; HS-SPME-GC-MS were used to analyze volatile components before and after decoction. Result： 48 volatile components were detected by SD-GC-MS, among them 28 were identified, accounting for 85.44% of the total volatile components. 122 volatile components were detected before the decoction, 73 were identified, accounting for 75.56% of the total volatile components. 50 volatile components were detected after the decoction, 27 were identified, accounting for 93.49% of the total volatile components. In three experiments a total of 97 peaks were identified; the highest content was α-asarone. Conclusion： This study not only provided a methodological foundation for volatile components analysis of Tiaoyuan powder, but also provided a reference on phamacodynamic material basis for improving autism.