罗汉果组培苗生产体系的建立

Establishment of Tissue Culture System for Siraitia grosvenorii

以罗汉果带节茎段为材料,对其诱导、继代、生根和移栽等条件进行研究,为罗汉果组培苗生产体系的建立提供条件。结果表明:MS+6-BA 1.0 mg·L-1+NAA 0.10 mg·L-1+蔗糖30g·L-1+琼脂6.0g·L-1为诱导培养基,诱导率可达40.0%;继代培养采用单节茎段扦插和促进腋芽生枝方法,适合的培养基为2/3改良MS+IBA 0.5mg·L-1+GA30.3mg·L-1+蔗糖40g·L-1+琼脂6.0g·L-1,增殖系数为4.0;生根培养基为1/2改良MS+IBA 0.5mg·L-1+蔗糖2.0%+活性炭0.1%+琼脂6.0g·L-1,生根率达到94%;栽培基质以泥炭土∶珍珠岩=10∶2为宜,移植成活率可达97.8%。

The stem segment of Siraitia grosvenorii was used as the material for establishing of tissue culture system with micropropogation.The results showed that the medium of MS ＋ 6-BA 1.0 mg·L-1 ＋ NAA 0.10 mg·L-1＋ sucrose 30 g·L-1 ＋ agar 6.0 g·L-1 was suitable for inducing axillary bud with induction rate of 40.0%. Single nodes cutting and accelerating for axillary buds growth were applied in subculture and optimal medium was 2/3 in modified MS ＋ IBA 0.5 mg · L-1 ＋ GA3 0.3 mg · L-1 ＋ sucrose 40 g · L-1 ＋ agar 6.0 g · L-1 ,with proliferation coefficient 4.0. The optimal rooting medium was 1/2 in modified MS ＋ IBA 0.5 mg·L-1 ＋ sucrose 2.0% ＋ AC 0.1 % ＋ agar 6.0 g·L-1 ,with rooting rate of 94%.The suitable transplanting medium was peat soil ＋ pearlite in the ratio of 10∶2,with survival rate for 97.8%.