摘要
利用生物显微技术对猞猁(Felis lynx)和豹猫(Prionailurus bengalensis)肝脏进行了比较,应用免疫组织化学方法检测了水通道蛋白-8(AQP-8)在肝脏中的表达情况.结果显示,与多数哺乳类相似,猞猁和豹猫的肝脏外被覆一层结缔组织薄膜,肝小叶的界限不清楚,中央静脉周围放射状排列着肝板、肝血窦及狄氏间隙,单层排肝细胞构成肝板,在门管区可清晰观察到小叶间静脉、小叶间动脉和小叶间胆管豹猫肝小叶的肝细胞排列较猞猁的规则,但肝细胞的直径较猞猁的小.猞猁肝细胞间比较松散,肝血窦较豹猫的发达.猞猁肝脏AQP-8的表达较豹猫的强烈.结果表明,猞猁和豹猫肝脏的组织结构及肝脏AQP-8在肝脏的表达与其生活习性相适应.
The liver structure of Felis lynx and Prionailurus bengalensis was compared with light microscope and expression of aquaporin - 8(AQP - 8)was measured by immunohistochemistry. The results show that the basic structure of the livers of Felis lynx and Prionailurus bengalensis was similar with the basic structure of most mammalian livers. The outer layer of the liver was a connective tissue capsule. The hepatic lobules were unclear. The hepatic cords,hepatic sinusoids and space of Disse arranged radially around a central vein and formed an ex-tensive spongework. The hepatic cords were comprised one row of hepatocytes. Interlobular artery,interlobular vein and interlobular bile duct in portal area were observed easily. The hepatic lobules of Prionailurus bengalensis were more regular than those of Felis lynx,but the diameter of hepatocytes of Prionailurus bengalensis was smaller than that of Felis lynx. The adjacent hepatocytes in the hepatic cords of Felis lynx were loose,the hepatic sinu-soids were very abundant. The expression of AQP - 8 in Felis lynx liver was stronger than that in Prionailurus ben-galensis liver. These indicate that the liver structure of Felis lynx and Prionailurus bengalensis and expression of AQP - 8 are adaptable to the life habit.
出处
《绵阳师范学院学报》
2015年第5期52-56,共5页
Journal of Mianyang Teachers' College
基金
甘肃省自然科学基金项目(1107RJZK243)
甘肃省高等学校科研资助项目(1128B-01)