银(Ⅲ)配合物-鲁米诺体系流动注射法测定醋酸泼尼松

Determination of Prednisone Acetate by Flow Injecting Ag(Ⅲ)Complexes-Luminol System

在碱性环境下,银（Ⅲ）配合物可与鲁米诺产生化学发光,醋酸泼尼松对该发光体系具有显著的增敏作用,据此提出了流动注射银（Ⅲ）配合物-鲁米诺化学发光体系测定醋酸泼尼松含量的方法。优化的试验条件如下：1鲁米诺溶液中氢氧化钠的浓度为0.6mol·L-1;2鲁米诺溶液的浓度为8.0×10-7 mol·L-1;3银（Ⅲ）配合物溶液中氢氧化钠的浓度为1.7mol·L-1;4银（Ⅲ）配合物溶液的浓度为5.0×10-5 mol·L-1。醋酸泼尼松的线性范围为6.0×10-8~8.0×10-5 mol·L-1,方法的检出限（3s/k）为2.9×10-9 mol·L-1。对1.0×10-6 mol·L-1醋酸泼尼松标准溶液连续测定11次,测定值的相对标准偏差为2.9%。加标回收率在100%~105%之间。

In alkaline medium, prednisone acetate could enhance the intensity of chemiluminescence produced by the reaction between Ag（ Ⅲ） complexes and luminol. Based on the fact, a method for determination of prednisone acetate by flow injecting Ag （Ⅲ） complexesquminol chemiluminescence system was proposed. The optimized conditions found were as follows： ① concentration of sodium hydroxide in luminol solution： 0. 6 mol·L-1 ②concentration of luminol solution： 8.0×10-7mol·L-1; ③ concentration of sodium hydroxide in Ag（ Ⅲ ） complexes solution： 1.7 mol·L-1 ; ④ concentration of Ag（ Ⅲ ） complexes solution： 5.0×10-5mol·L-1. Linearity range of prednisone acetate was found between 6. 0 × 10-8 and 8. 0 × 10-5 mol·L-1 with detection limit （3s/k） of 2. 9 × 10-9mol·L-1. 1.0× 10-6mol·L-1 prednisone acetate standard solution was tested for 11 measurements, value of RSD found was 2.9%. Values of recovery found were in the range of 100%-105%.