柚皮苷的大鼠血浆蛋白结合率研究

Study on plasma protein binding rate of naringin in rats

目的研究柚皮苷的大鼠血浆蛋白结合率。方法使用平衡透析法模拟柚皮苷在体内与血浆蛋白结合的过程,并建立测定柚皮苷的高效液相色谱-质谱联用(HPLC-MS/MS)方法,测定低、中、高3个浓度(100、600、3 600 ng/m L)的柚皮苷在透析袋内血浆中和透析袋外缓冲液中的浓度,计算血浆蛋白结合率。结果低、中、高3个浓度的柚皮苷在体外与大鼠的平均血浆蛋白结合率分别为91.5%、90.5%和96.3%,3个浓度之间的血浆蛋白结合率比较差异无统计学意义(P>0.05)。结论柚皮苷与大鼠血浆蛋白高度结合。

Objective To study the plasma protein binding rate of naringin in rats. Methods Under three dif-ferent concentrations （100,600 and 3 600 ng/mL）,this paper used equilibrium dialysis method to imitate the binding process between naringin and the plasma protein of rats,and determined the concentration of naringin in and out of the dialysis membrane by high performance liquid chromatography-tandem mass spectrometry （ LC-MS/MS） method. The binding rate was calculated. Results Under the experimental concentrations,the plasma protein binding rates of naring-in in rats were 91. 5%,90. 5% and 96. 3% at low,middle and high concentrations,respectively. There was no signifi-cant difference among the three concentrations. Conclusion Naringin is highly bound to plasma proteins in rats.