一水肌酸对杜浙猪肉质性状、肌肉磷酸原代谢、纤维类型特征及蛋白质溶解度的影响

Effects of Creatine Monohydrate on Meat Quality,Phosphagen Metabolism,Myofiber Types and Protein Solubility in Longissimus Muscle of Duroc × Zhongbai(DZ) Crossed Pigs

本文旨在研究饲粮添加一水肌酸(CMH)对杜浙猪肉质性状、肌肉磷酸原代谢、纤维类型特征及蛋白质溶解性的影响。试验选取体重70 kg左右杜浙猪30头,随机分为2组,每组5个重复,每个重复3头猪。对照组饲喂基础饲粮,试验组饲喂基础饲粮+0.5%CMH,试验期30 d。结果显示:与对照组相比,饲粮添加CMH对杜浙猪生长性能无显著影响(P>0.05),使背最长肌宰后24 h p H和剪切力显著增加(P<0.05),滴水损失和压榨损失显著降低(P<0.05),背最长肌的肌酸(Cr)总量、磷酸肌酸(PCr)储备量、肌酸激酶(CK)活性、白蛋白及肌原纤维蛋白溶解度显著增加(P<0.05),背最长肌肌球蛋白重链(My HC)Ⅱa mRNA比例显著降低(P<0.05)。结果提示,饲粮添加CMH对杜浙猪肉质性状具有调控作用,对肌肉磷酸原代谢能力、蛋白质溶解度及肉品系水力产生显著影响。

This experiment was conducted to investigate the effects of dietary creatine monohydrate on meat quality, phosphagen metabolism, myofiber types and protein solubility in longissimus muscle of DurocxZhong- bai （DZ） crossed pigs. Thirty DZ crossbred pigs （ about 70 kg bodyweight） were divided into two groups with five replicates per group and three pigs per replicate. Pigs in the control group were fed the basal diet, and the others were fed the basal diet＋0.5% creatine monohydrate （CMH）. The experiment lasted for 30 days. The re- sults showed that compared with the control group, dietary CMH had no influence on growth performance of DZ crossed pigs （P〉0.05）, the pH at 24 h postmortem and shear force of longissimus muscle were significant- ly increased （P〈0.05）, the drip loss and milling loss of longissimus muscle were significantly decreased （P〈 0.05） , the creatine （Cr） and phosphocreatine （PCr） content, creatine kinase （CK） activity, total protein and myofibrilla protein solubilities of longissimus muscle were significantly increased （P〈0.05）, the myosin heavy chain （MyHC） Ⅱ a mRNA ratio of longissimus muscle was significantly decreased （P〈0.05）. In conclusion, dietary CMH can regulate meat quality, and it has significant effect on phosphagen metabolism capacity, pro- tein solubility and water hold capacity of longissimus muscle.