湿迪胶囊的免疫调节作用研究

Study on the immunoregulation effects of the Shidi capsule

目的研究湿迪胶囊的免疫调节作用。方法通过二硝基氟苯(DNFB)诱导迟发型变态反应模型检测耳肿胀度,碳粒廓清法测定网状内皮系统吞噬功能,分光光度法测定血清溶血素,噻唑蓝(MTT)法测定脾淋巴细胞增殖反应。结果湿迪胶囊高、中剂量对二硝基氟苯致小鼠迟发型超敏反应实验中测得的耳肿胀度均有明显抑制效果;湿迪胶囊高剂量能显著提高吞噬指数K,湿迪胶囊高、中剂量能校正吞噬指数α;湿迪胶囊高、中剂量组小鼠血清中溶血素的含量明显降低;湿迪胶囊各剂量可以抑制小鼠的脾淋巴细胞增殖反应。结论湿迪胶囊可能对小鼠免疫功能具有双向调节作用,其治疗类风湿性关节炎的作用可能与抑制特异性细胞免疫功能与抑制体液免疫功能有关,同时具有提高小鼠的非特异性免疫功能。

Objective To investigate the immunoregulation effects of the Shidi capsule.Methods The delayed type hypersensitivity （DTH）mouse model induced by dinitrofluorobenzene （DNFB）was used to study the effects of the Shidi capsule on the levels of DTH;phagocytosis of mononuclear macrophage was determined by cleaning carbon particle method;spectrophotograhphy was used to estimate levels of serum hemolysin;splenic lymphocytes proliferation was measured by MTT method.Results Shidi capsule 9.00 and 4.50 g· kg-1 can inhibit delayed type hypersensitivity of mice significantly.Shidi capsule increased the rates of clearance and clearance index in Carbon Clearance Test.hidi capsule 9.00 and 4.50 g·kg-1 dose could decrease the content of serum hemolysin.In addition,it could improve the proliferation of splenic lymphocytes.Conclusions Our results indicated that Shidi capsule can inhibit specific cellular im-munity and humoral immunity.