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Kinetic study of all-or-none hemolysis induced by cationic amphiphilic polymethacrylates with antimicrobial activity

Kinetic study of all-or-none hemolysis induced by cationic amphiphilic polymethacrylates with antimicrobial activity
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摘要 To gain an understanding of the toxicity of antimicrobial polymers to human cells, their hemolytic action was investigated using human red blood cells (RBCs). We examined the hemolysis induced by cationic amphiphilic methacrylate random copolymers, which have amino ethyl sidechains as cationic units and either butyl or methyl methacpjlate as hydrophobic units. The polymer with 30 tool% butyl sidechains (B3o) displayed higher hemolytic toxicity than the polymer with 59 tool% methyl sidechains (Ms9). B3o also induced faster release of hemoglobin from RBCs than lVlsg. A new theoretical model is proposed based on two consecutive steps to form active polymer species on the RBC membranes, which are associated to RBC lysis. This model takes the all-or-none release of hemoglobin by the rupture of RBCs into account, providing new insight into the polymer-induced hemolysis regarding how individual or collective ceils respond to the polymers. To gain an understanding of the toxicity of antimicrobial polymers to human cells, their hemolytic action was investigated using human red blood cells (RBCs). We examined the hemolysis induced by cationic amphiphilic methacrylate random copolymers, which have amino ethyl sidechains as cationic units and either butyl or methyl methacpjlate as hydrophobic units. The polymer with 30 tool% butyl sidechains (B3o) displayed higher hemolytic toxicity than the polymer with 59 tool% methyl sidechains (Ms9). B3o also induced faster release of hemoglobin from RBCs than lVlsg. A new theoretical model is proposed based on two consecutive steps to form active polymer species on the RBC membranes, which are associated to RBC lysis. This model takes the all-or-none release of hemoglobin by the rupture of RBCs into account, providing new insight into the polymer-induced hemolysis regarding how individual or collective ceils respond to the polymers.
出处 《Chinese Chemical Letters》 SCIE CAS CSCD 2015年第4期479-484,共6页 中国化学快报(英文版)
基金 supported by the Department of Biologic and Materials Sciences,University of Michigan School of Dentistry,NSF CAREER Award (No.DMR-0845592 to KK) JSPS KAKENHI,Grant-in-Aids for Challenging Exploratory Research (No.25650053) Young Scientists (Nos.24681028 and 22700494 to KY)
关键词 HemolysisAntimicrobial polymerAmphiphilic polymethacrylateKinetic analysisMembrane disruption HemolysisAntimicrobial polymerAmphiphilic polymethacrylateKinetic analysisMembrane disruption
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  • 1H,W, Boucher, G.H. Talbot, J.S. Bradley, et al., Bad bugs, no drugs: no ESI(APE! An update from the Infectious Diseases Society of America, Clin. Infect. Dis. 48 (2009) 1-12.
  • 2K. Kuroda, W. DeGrado, Amphiphilic polymethacrylate derivatives as antimicro- bial agents, J. Am. Chem. Soc. 127 (2005) 4128-4129.
  • 3H. Takahashi, E.F. Palermo, K. Yasuhara, G.A. Caputo, K. Kuroda, Molecular design, structures, and activity of antimicrobial peptide-mimetic polymers, Macromol. Biosci. 13 (2013) 1285-1299.
  • 4K. Kuroda, G.A. Caputo, Antimicrobial polymers as synthetic mimics of host- defense peptides, Wires Nanomed. Nanobiotechnol. 5 (2013) 49-66.
  • 5E.F. Palermo, S. Vemparala, K. Kuroda, Cationic spacer arm design strategy for control of antimicrobial activity and conformation of amphiphilic methacrylate random copolymers, Biomacromolecules 13 (2012) 1632-1641.
  • 61. Sovadinova, E.F. Palermo, M. Urban, et al., Activity and mechanism of antimi- crobial peptide-mimetic amphiphilic polymethacrylate derivatives, Polymers 3 (2011) 1512-1532.
  • 7w. van't Hof, E. Veerman, g.]. Helmerhorst, A. Amerongen, Antimicrobial peptides: properties and applicability, Biol. Chem. 382 (2001) 597-619.
  • 8G.E. Rowe, R.A. Welch, Assays of hemolytic toxins, Methods Enzymol. 235 (1994) 657-667.
  • 9K. Kuroda, G.A. Caputo, W.F. DeGrado, The role of hydrophobicity in the antimi- crobial and hemolytic activities of polymethacrylate derivatives, Chem. Eur. J. 15 (2008) 1123-1133.
  • 10I. 5ovadinova, E.F. Palermo, R. Huang, L.M. Thoma, K. Kuroda, Mechanism of polymer-induced hemolysis: nanosized pore formation and osmotic lysis, Bio- macromolecules 12 (2011 ) 260-268.

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