细粒棘球绦虫14-3-3zeta蛋白的生物信息学分析

Application of bioinformatic analysis in 14-3-3zeta protein of Echinococcus granulosus

目的应用生物信息学技术对细粒棘球绦虫(Echinococcus granulosus)14-3-3zeta蛋白的结构和功能进行预测和分析,为进一步的实验研究提供依据。方法利用美国国家生物技术信息中心(NCBI,http://www.ncbi.nlm.nih.gov/)和瑞士生物信息学研究所的蛋白分析专家系统(ExPASY,http://expasy.org/)提供的各种有关基因和蛋白序列、结构信息分析的工具,并结合其它生物信息学分析软件,对该蛋白质的结构和功能进行预测和分析。结果该基因全长为771 bp,编码256个氨基酸,其编码的蛋白相对分子量理论预测值和等电点分别是29.4 kDa和5.04。预测该蛋白无信号肽和跨膜区,二级结构含8个α-螺旋和12个β-折叠股,氨基酸序列中有9个潜在抗原表位。结论初步认识了细粒棘球绦虫14.3-3zeta蛋白的基本特征,为深入研究该蛋白的生物学功能奠定了基础。

Objective To predict and analyze the structure and function of 14-3-3zeta protein from Echinococcus granulosus by bioinformatics technology. Methods The structure and function of Eg]4- 3-3zeta protein was identified from two biological information sites, USA National Center for Biotechnology Information （NCBI, http：//www.ncbi.nlm.nih.gov/）, and Expert System for analysis of protein of the Swiss Institute of bioinformatics （ExPASY,http：//expasy.org/）, which offer the analysis of various related gene and protein sequence, structure information tools, and other bioinformatics analysis software. Results The full- length cDNA sequence encoding Eg14-3-3zeta included a complete open reading frame （ORF） of 771 bp coding to a putative protein with 256 amino acids. Molecular weight of Egl4-3-3zeta was predicted to be 29.4 kDa and its isoelectric point was 5.04. The protein had no signal peptide site and transmembrane do- main. Secondary structure of Egl4-3-3zeta contained 8 alpha-helices and 12 beta-strands.There were 9 po- tential epitopes in the sequence of amino acids of Egl4-3-3zeta. Conclusion The characteristics of 14-3- 3zeta protein from Echinococcus granulosus was identified.