基于SSR标记和骨干自交系的外引玉米自交系杂种优势群划分

Heterotic grouping of exotic maize inbred lines by SSR markers and elite maize inbred lines

研究玉米杂种优势群及其模式对于提高育种效率及预见性具有重要意义.利用40个SSR核心标记、6份骨干自交系和49份外引自交系进行杂种优势群划分和遗传多样性分析,结果表明,40个SSR标记在49份外引自交系中共检测到242个等位变异,每个标记检测到4～11个等位基因、平均6.05个,PIC变化范围0.3224 （phi072） ～0.8376 （bnlg2235）、平均为0.6347,基因多样性趋势与PIC基本一致,变化范围0.3640（phi072） ～0.8538 （bnlg2235）、平均为0.6767.结合骨干自交系系谱的聚类与基于混合模型的群体结构分析结果,将外引材料划分为唐四平头、瑞德、改良瑞德、兰卡斯特4个类群.

It is significant for improving the efficiency and predictability of maize breeding to study heterotic grouping of maize. In this study,40 simple sequence repeat（SSR）primers and 6 elite maize inbred lines were used to detect genetic variation and heterotic grouping among 49 exotic inbred lines. A total of 242 alleles was detected with a range between 4 to 11 and an average of 6.05 alleles per locus. The PIC and gene diversity showed the similar trend,varied from 0.3224（phi072）to 0.8397（bnlg2235）and 0.3640（phi072）-0.8538（bnlg2235）,the averages were 0.6347 and 0.6767. Inbred lines were clustered into 4 groups referring to the pedigree of leading self lines,including Tangsipingtou,Reid,Improved Reid and Lancaster group.