苯并[a]芘对小鼠睾丸支持细胞缝隙连接蛋白表达及细胞增殖的影响

Effects of Benzo[a]pyrene on Expression of Junction Protein and Proliferation in Mouse Sertoli Cells

[目的]探讨苯并[a]芘(Ba P)在不引起小鼠睾丸支持细胞凋亡的情况下,对缝隙连接蛋白43(CX43)表达及细胞增殖的作用。[方法]取小鼠睾丸支持细胞系——TM4细胞,以二甲基亚砜为对照,0.5、10.0μmol/L浓度的Ba P同时染毒培养的TM4细胞,并于染毒的4 h、72 h后收集细胞,检测细胞的活力、增殖、凋亡情况,以及CX43的m RNA和蛋白的表达量。[结果]与对照组相比:Ba P染毒4 h时,两个染毒组细胞存活率、增殖、凋亡和CX43蛋白表达没有明显变化(P>0.05),而CX43 m RNA的表达升高(P<0.05);72 h时,两个染毒组细胞凋亡无明显变化,而细胞存活率和增殖均下降(P<0.05),CX43 m RNA和蛋白表达出现上升(P<0.05)。[结论]0.5、10.0μmol/L浓度的Ba P染毒小鼠睾丸支持细胞,可抑制细胞增殖,引起CX43 m RNA和蛋白表达升高。

[Objective] To investigate the effects of benzo[a]pyrene（Ba P） on the expression of connexin 43（CX43） and cell proliferation in mouse sertoli cells without change of cell apoptosis. [Methods] The mouse sertoli cell line TM4 cells were exposed to varied concentrations of Ba P（dimethyl sulfoxide control group, 0.5 and 10.0 μmol/L Ba P groups） for 4 h or 72 h. Then these cells were collected to measure cell viability, proliferation, and apoptosis. Besides, m RNA and protein expression levels of CX43 were also measured. [Results] Compared with the control group, no difference in the viability, proliferation, apoptosis, or protein expression levels of CX43 was found in the TM4 cells with 4 h-exposure to 0.5 and 10.0 μmol/L Ba P（P〉0.05）, but the m RNA expression levels of CX43 was significantly increased（P〈0.05）. For 72 h exposure, the viability and proliferation were significantly decreased（P〈0.05） in the TM4 cells without change of apoptosis, and the m RNA and protein expression levels of CX43 were significantly increased（P〈0.05）. [Conclusion] Ba P exposure at 0.5 and 10.0 μmol/L could inhibit proliferation of mouse sertoli cells and increase m RNA and protein expression levels of CX43.