热休克蛋白70在脊髓损伤患者血清中的表达及临床意义

Temporal expression profile of HSP70 in serum of patients with spinal cord injury and its clinical significance

目的探讨热休克蛋白70(HSP70)在脊髓损伤患者血清中的动态表达以及其表达量与脊髓损伤严重程度的关系。方法入选76例脊髓损伤患者为试验组,30例健康成年人为对照组,分别于脊髓损伤后6,12,24,72,120 h静脉采血,免疫印迹法检测患者血清中HSP70的表达量,并研究其表达量与患者损伤程度的关系。结果与对照组相比,试验组脊髓损伤患者血清中HSP70的表达量在损伤后6 h显著升高(P<0.05),在损伤后24 h达到峰值(P<0.05),72,120 h时逐渐降低。HSP70的表达量与脊髓损伤的严重程度存在显著正相关,损伤程度越重的患者HSP70表达量越高(P<0.05)。结论 HSP70在脊髓损伤患者血清中的表达量显著升高,有可能对早期评估脊髓损伤严重程度具有重要的意义。

Objective To investigate the expression of serum heat shock protein 70 （ HSP70 ） in patients with spinal cord injury and its correlation with the extent of injury severity .Methods There were 76 patients with spinal cord injury in experiment group , and 30 healthy volunteers enrolled in control group.Blood samples were obtained at 6, 12, 24, 72 , 120 h after spinal cord injury in 76 patients.Western blot analysis was used to determine the expression of HSP 70 and the correlation between HSP70 level and the extent of injury severity was also evaluated . Results The patients with spinal cord injury showed a higher expression of HSP70 than control group at 6 h, and peaked at 12 h.But the expression decreased 72 , 120 h post spinal cord injury （ P〈0.05 ） . Furthermore , the level of HSP70 was positively correlated with the extent of injury severity in spinal cord injury patients （ P 〈0.05 ） . Conclusion Increased serum HSP70 level may constitute an early predictor of unfavorable outcome in spinal cord injury patients .