薏苡茎水提取物对H22荷瘤小鼠的抗肿瘤作用研究

Anti-tumor effect of water extract from stem of Coix Lachryma-jobi L.in mice

目的研究薏苡茎水提取物对荷瘤小鼠的抑瘤作用及对免疫器官的影响。方法以肝癌H22荷瘤小鼠作为实验动物模型,将实验小鼠随机分成7组,模型组、环磷酰胺组、5个剂量实验组,分别给予0.9%氯化钠、环磷酰胺(0.02g·kg-1)、薏苡茎水提取物(2,4,6,8,10 g·kg-1)灌胃给药。连续9 d后,颈椎脱臼处死,称重并剖离肿瘤、肝、胸腺、脾,计算体质量、抑瘤率及肝、胸腺、脾指数。结果与模型组比较,薏苡茎水提取物对肝癌H22具有明显的抑制作用(P<0.05),其对肝指数、体重增长也有明显的影响(P<0.05)。但其对荷瘤小鼠的胸腺、脾指数无明显影响。结论薏苡茎水提取物具有抗肿瘤作用。

Objective To study the tumor inhibitory effects and an in-fluence on immune organs of the water extract from stem of Coix Lachry-ma-jobi L.（WESCLL） in mice with liver cancer H22.Methods The experimental subjects were using mice with liver cancer H 22.The mice are randomly divided into 7 groups and were fed 0.9%sodium chloride , cyclophosphamide （0.02 g· kg -1 ）, WESCLL（2,4,6,8,10 g· kg -1 ） by oral gavages.After 9 days′administration, the mice were killed. Their subcutaneous sarcoma ,liver,thymus and spleen were separated and weighted.Then the tumor inhibiting rates and liver , thymus and spleen index were calculated.Results The WESCLL has obvious effects on re-straining the liver cancer H22.There was significant difference compared with model group （P〈0.05）.The effect of WESCLL is more noticeable on liver index and weigh′s increase of the mice （ P〈0.05 ） .There was not significant difference on thymus and spleen indexes compared with model group （ P〈0.05 ） .Conclusion WESCLL have distinct effect on anti-tumor.