生脉饮对糖尿病大鼠心肌病变及脂质代谢的作用研究

Effect of Shengmai Solvent on myocardial fibrosis and lipid metabolism in rat model of diabetic cardiomypathy

目的观察生脉饮对糖尿病心肌纤维化以及脂质代谢的作用。方法将72只雄性Wistar大鼠随机分成6组,对照组、模型组、预防组和高中低3个剂量实验组。对照组和模型组,均给予饮用水12 m L·kg-1;预防组,实验开始时灌胃给予生脉饮12 m L·kg-1;高、中、低3个剂量实验组,在造模成功后,分别灌胃给予12,6,3 m L·kg-1生脉饮。除对照组以外,其余各组喂以高脂高热量饲料。4周后,给予1次性腹腔注射链脲佐菌素35 mg·kg-1。继续喂养1周后,测定空腹血糖,连续2次血糖≥16.7 mmol·L-1,且有多饮、多食、多尿的大鼠,用于后期实验。15周时,检测血糖血脂,剖杀,心脏称重,光学显微镜下观察心肌的病理变化,用RT-PCR方法检测心肌胶原的含量。结果预防组和实验组的心脏系数均明显低于模型组,高密度脂蛋白、载脂蛋白比值均明显高于模型组。预防组,低密度脂蛋白明显低于模型组。生脉饮能减少糖尿病心肌病大鼠心肌胶原的表达,但差异无统计学意义。结论生脉饮减轻糖尿病心肌病大鼠的心肌纤维化进程可能与其能改善脂质代谢有关。

Objective To explore the effect of Shengmai Solvent on myocardial fibrosis in rat model of diabetic cardiomypathy . Methods Wistar rats of 72 were randomly divided into 6 groups, including control group , model group , prevetion group , low -dose , mid-dose and high -doses of test groups. Shengmai Solvent was administered via gavage. Prevetion group （ Shengmai Solvent , 12 mL· kg -1 ） was administered at the beginning . Low -dose （ 3 mL· kg -1 ） , mid -dose （ 6 mL · kg -1 ） and high -dose （ 12 mL· kg -1 ） groups were administered with Shengmai Solvent at 5th week. All groups except control group were fed on high -cholestrerol diet for 4 weeks with intraperitoneal injection of streptozotocin （ 35 mg· kg -1 ） . One week later , blood glucose is checked , and those with two consecu-tive blood glucose ≥16.7 mmol· L-1 .The blood glucose , lipid were detected at 15 th weeks after administration .The rats were sacrificed for determination of heart wet weight . Cardiac collagen was detected by RT-PCR.Results Compared with model group , the heart weight /body ratio were significantly decreased in the Shengmai groups .The level of HDL and ApoAI /ApoB were significantly increased in Shengmai;groups.The level of LDL in prevetion group was decreased significantly .Conclusion These results indicate that the mechanism of Shengmai reduced the progression of DCM might be related to alteration of lipid metabolism .