益气化瘀方对产后子宫复旧不全大鼠一氧化氮和一氧化氮合酶的影响

Effects of Yiqi Huayu Formula on Nitric Oxide and Nitric Oxide Synthase in Rat Models with Postpartum Subinvolution of Uterus

目的探讨益气化瘀方促进产后子宫复旧的作用机制。方法选取50只雌性SD大鼠,采用阴道内注射大肠杆菌制备产后子宫复旧不全模型。按妊娠先后次序编号,随机分为空白组、模型组、新生化组及益气化瘀方低、高剂量组,空白组和模型组灌胃纯净水,益气化瘀方低、高剂量组给予益气化瘀方灌胃,新生化组给予新生化颗粒灌胃,每日1次,连续7 d。HE染色观察子宫内膜形态,硝酸还原酶法检测大鼠血清中一氧化氮(NO)、一氧化氮合酶(NOS)的含量。结果模型组大鼠子宫组织切片HE染色示黏膜下层充血、出血,内膜连续性中断,伴炎性细胞浸润,提示造模成功。模型组NO、NOS含量均高于空白组;与模型组比较,各给药组NO、NOS明显降低(P<0.05,P<0.01);与新生化组比较,益气化瘀高剂量组NO、NOS降低(P<0.05)。结论益气化瘀方通过降低NO、NOS水平,抑制子宫螺旋动脉异常舒张,促进子宫内膜的重建与修复。

Objective To explore the action mechanism of Yiqi Huayu Formula for postpartum subinvolution of uterus. Methods Fifty female SD rats were selected model with postpartum subinvolution of uterus was made by injecting escherichia coli in vagina. Rats were randomly divided into 5 groups according to pregnancy time：blank group, model group, Xinshenghua group, and Yiqi Huayu high and low dose groups. Blank group and model group received gavage with water;Yiqi Huayu low and high dose groups received gavage with Yiqi Huayu Formula;Xinshenghua group received gavage with Xinshenghua Granule, once a day for 7 days. Uterine endometrial morphology was observed by HE staining. The contents of NO and NOS were respectively detected by Nitrate reductase method. Results Uterus submucosal hyperemia, bleeding, and endometrial discontinuity with the infiltration of inflammatory cells of rats in the model group suggested that the models were successfully established. Compared with blank group, the contents of NO and NOS in model group were higher significantly. Compared with model group, the contents of NO and NOS significantly decreased in both Xinshenghua group and Yiqi Huayu high and low groups （P〈0.01, P〈0.01）. Compared with Xinshenghua group, Yiqi Huayu high dose group was lower （P〈0.05）. Conclusion Yiqi Huayu Formula could promote reconstruction and repair of the endometrium by reducing the contents of NO and NOS and inhibiting abnormal uterine spiral arteries relaxation.