甘肃不同产地黄芩茎叶和根对急性肝损伤小鼠的影响

Effect of the Stems and Leaves and Roots of Scutellariae Radix from Different Producing Areas of Gansu Province on Mice with Acute Liver Injury

目的观察甘肃不同产地黄芩茎叶、根对急性肝损伤小鼠的影响,探讨黄芩茎叶和根保肝作用的差异。方法将昆明种小鼠随机分为正常组、模型组、阳性对照组及甘肃陇西、岷县、渭源、漳县产黄芩根和茎叶组。各组分别给予相应药物灌胃,每日1次,连续7 d。末次给药2 h后,采用腹腔注射0.1%CCl4橄榄油溶液诱导肝损伤模型。造模18 h后,计算肝系数,检测血清谷氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)活性及肝组织总抗氧化能力(T-AOC)水平。结果与模型组比较,不同产地黄芩根组小鼠肝系数及血清中ALT、AST活性显著降低(P<0.05),肝组织T-AOC水平升高(P<0.05);同一产地根与茎叶组比较,各黄芩根组小鼠肝系数及血清中ALT、AST活性低于黄芩茎叶组(P<0.05),肝组织T-AOC水平高于黄芩茎叶组(P<0.05)。结论黄芩根水煎液对CCl4诱导的急性肝损伤具有保护作用,而黄芩茎叶水煎液对急性肝损伤保护作用不明显。

Objective To explore liver protective effects of the stems and leaves and roots of Scutellariae Radix from different areas of Gansu Province;To discuss differences in liver protection of the stems and leaves and roots of Scutellariae Radix. Methods All Kunming mice were randomly divided into 11 groups：normal group, model group, positive control group, Scutellariae Radix root groups and Scutellariae Radix stem-leaf groups of Longxi, Minxian, Weiyuan, Zhangxian. The mice received gavage with relevant medicine once a day for 7 days. 2 hours after the last medication, the mice were given peritoneal injection with 0.1%CCl4 and olive oil to establish the models with liver injury. Hepatic index, the activity of serum ALT and AST, the levels of T-AOC in the liver tissue were calculated in 18 hours after establishing the models. Results Compared with the model group, hepatic index, the activity of serum ALT and AST in the mice of Scutellariae Radix root groups from difference producing areas decreased significantly （P〈0.05）;the levels of T-AOC in the liver tissue was raised notably （P〈0.05）. Compared with stem-leaf groups from the same producing area, hepatic index, the activity of ALT and AST in the mice of Scutellariae Radix root groups were remarkably lower than these of Scutellariae Radix stem-leaf groups （P〈0.05）;the levels of T-AOC in the liver tissue was evidently higher than that of Scutellariae Radix stem-leaf group （P〈0.05）. Conclusion Scutellariae Radix root water decoction can prevent and protect CCl4-induced acute liver injury to a certain degree, while Scutellariae Radix stem-leaf water decoction shows no significant protective effect on acute liver injury.