甲强龙对实验性变态反应性脑脊髓炎小鼠的协同刺激分子CD80和CD86的影响

Effect of methylprednisolone on the levels of CD80 and CD86 in mice with experimental autoimmune encephalomyelitis

目的探讨甲强龙对实验性变态反应性脑脊髓炎小鼠的协同刺激分子CD80和CD86的影响。方法实验性变态反应性脑脊髓炎(EAE)模型通过以髓鞘少突胶质细胞糖蛋白多肽35-55(MOG35-55)为抗原诱导。将30只雌性C57BL/6J小鼠随机分为甲强龙组、EAE组和正常组,每组10只。采用蛋白质印迹法检测大脑的CD80和CD86表达水平。结果甲强龙组神经功能缺损症状轻于EAE组,大脑CD80表达水平明显低于EAE组(P<0.05),而CD86表达明显高于EAE组(P<0.05)。结论甲强龙能改善EAE小鼠的症状,其作用机制之一是抑制CD80表达并上调CD86的表达。

Objective To explore the effect of methylprednisolone on the levels of cluster of differentiation （CD）80 and CD86 in the central nervous system of mice with experimental autoimmune encephalomyelitis（EAE）. Methods The EAE model was induced in mice by immunized with myelin oligodendrocyte glycoprotein peptides （MOG35-55）.30 female C57BL/6J mice were randomly divided into 3 groups： methylprednisolone-treated group, EAE group and normal group.The brain tissues were collected and their expressions of CD80 and CD86 were detected using western blot.Results The neurological deficits of the methylprednisolone-treated mice were lighter than those of the EAE mice（P〈0.05）, while the methylprednisolone-treated mice displayed the lower expression of CD80 and the higher expression of CD86 than the EAE mice did（P〈0.05）.Conclusion Methylprednisolone can protect a-gainst EAE in mice by the inhibition of CD80 and upregulation of CD86.