摘要
目的探讨血管紧张素Ⅱ受体拮抗剂厄贝沙坦对糖尿病大鼠肾脏chemerin的影响。方法将SD大鼠随机分为对照组、糖尿病组和厄贝沙坦治疗组,以链脲佐菌素(STZ)及高糖高脂饮食制备2型糖尿病大鼠模型,给予厄贝沙坦40mg/(kg·d)治疗8周后处死大鼠。Western blot检测各组肾组织chemerin蛋白的表达。应用放射免疫法检测血清血管紧张素Ⅱ(AngⅡ)的含量。结果与对照组相比,糖尿病组血肌酐、尿素氮、24小时尿白蛋白排泄率及AngⅡ水平均显著升高(P<0.05),厄贝沙坦治疗后血肌酐及24小时尿白蛋白排泄率较糖尿病组显著降低(P<0.05)。糖尿病组大鼠肾组织中chemerin蛋白表达明显高于对照组,治疗组表达水平明显下降。结论 chemerin在糖尿病大鼠肾脏中表达增加,厄贝沙坦可能通过抑制RAS系统降低chemerin的表达,从而对肾脏起保护作用。
Objective To study the effects of renin-angiotensin system （RAS）on the expression of chemerin in streptozotocin-induced diabetic rats.Methods After 6-week feeding with high-fat and high-carbohydrate diet,rats were administrated with STZ and randomized to control,diabetic,irbesartan-treated groups.The chemerin protein was identified by western blot.The level of angiotensin Ⅱ （AngⅡ ）was measured by radioimmunoassay.Results Blood urea nitrogen （BUN）,serum creatinine （Scr）,urinary albumin excretion rate（UAER）and AngⅡ levels in diabetic group were much higher than that in the control group.The level of chemerin in the renal tissues was significantly elevated in the diabetic group compared with the control group.Eight weeks after Irbesartan treatment,the Scr,UAER and chemerin were significant decreased.Conclusion Irbesartan treatment ameliorate renal function through an inhibitory effect on the activation of intrarenal RAS,which may impact chemerin expression levels in the kidney of diabetic rats.
出处
《中国实验诊断学》
2015年第5期697-699,共3页
Chinese Journal of Laboratory Diagnosis
基金
黑龙江省教育厅科学技术研究项目资助(编号:12531233)
