细粒棘球蚴感染中阻断TGF-β1受体对淋巴细胞的影响

Effect of inhibition of TGF-β1 receptors on lymphocytes during infection of Echinococcus granulosus

目的:观察TGF-β1对细粒棘球蚴体外与BALB/c小鼠T淋巴细胞培养的影响。方法:阻断剂组:BALB/c小鼠脾细胞+细粒棘球蚴+SB525334共培养;对照组:BALB/c小鼠脾细胞+细粒棘球蚴+PBS共培养;空白对照:BALB/c小鼠脾细胞+RPMI-1640培养基+SB525334共培养。方法:48 h收集淋巴细胞,流式细胞仪检测T淋巴细胞亚群、CD4+CD25+T细胞的数量、NK细胞的数量及其活性受体NKG2D的表达;乳酸脱氢酶法检测NK细胞的杀伤活性。结果:体外阻断TGF-β1受体后,引起细粒棘球蚴介导的CD4+T细胞数量升高、CD8+T细胞数量降低、CD4+/CD8+T细胞比值升高、CD4+CD25+T细胞数量减少、NK细胞活性受体NKG2D的表达增加、NK细胞对靶细胞Yac-1的裂解率增加和NK细胞活性及其活性受体NKG2D成正相关。结论:体外阻断TGF-β1受体可提高T淋巴细胞免疫,从而抵制细粒棘球蚴的感染。

Objective：To investigate the effects of TGF-131 on T lymphocytes of BALB/c mice infected with Echinococcus granulosus（ E. granulosus） in vitro. Methods： The inhibitor group： the spleen cells of BALB/c mouse were co-cultured with E. granulosus and SB525334. The control group：the spleen cells of BALB/c mouse were co-cultured with E. granulosus and PBS. The blank group：the spleen cells of BALB/c mouse were co-cultured with RPMI-1640 medium and SB525334. The lymphocytes were collected at 48 h post-infection. The T lymphocyte subsets, the number of CD4＋ CD25＋ T cells, the number of NK cells, and the expression of NKG2D receptor were detected by flow cytometry. The NK cell activity was determined with the lactate dehydrogenase leakage assay（LDH）. Results： The inhibition the TGF-IM receptors resulted in the increase of in the number of CD4＋ T cells, the decrease in the number of CD8＋ T cells, the increase of in the ratio CD4＋/CD8＋ T cells, the decrease of in the number of CD4＋ CD25 ＋ T cells, the increase in the expression of the NKG2D receptors, the increase in the lysis rate of Yac-1 ceils by NK cells, and a positive cor- relation between the expression of activity receptor NKG2D and killing activity of NK, which were mediated by E. granulosus. Conclusion： The inhibition of TGF-1β receptors can enhance the immune response of T lymphocytes against E. granulosus infection in vitro.