铜胁迫下小麦幼根转录组学及蛋白质组学研究

Transcriptomics and Proteomics Analysis in Root of Wheat under Copper Stress

为探讨外源铜胁迫对小麦幼根的影响,采用水培方法,测定0~60 mg/L不同质量浓度铜处理下小麦幼苗生长发育、转录组表达和蛋白质组表达的差异,探索其抵御重金属毒害的分子机制。结果表明：高质量浓度的铜影响小麦生长,随胁迫时间的延长,小麦幼苗萎蔫发黄,幼根生长受抑。小麦幼根转录组测序检测,共得到2 283个差异表达基因（DEGs）,其中826个DEGs表达上调,1 457个DEGs表达下调;将DEGs进行KEGG pathway注释,被注释到31个代谢途径中。双向电泳试验检测,小麦幼根中有2 049个蛋白质点,30 mg/L铜处理96 h后,与对照（0 mg/L处理）相比,约130个蛋白质点表达出现显著差异,其中56个丰度增加,74个丰度降低;质谱鉴定部分差异表达蛋白,抗性蛋白如谷胱甘肽转移酶、27K蛋白等在胁迫下表达上升,而生理代谢相关蛋白表达降低。

In order to explore the influence of exogenous copper（Cu） on roots of wheat,the solution cul-ture experiment was carried out to study the growth index,transcriptomics and proteomics differential ex-pression of wheat root. The results indicated that the growth of wheat was inhibited significantly,the influ-ence were closely related to Cu dose and time of treatment. The transcriptomics were analyzed,and 2 283 differential expression genes（DEGs） were detected,of which 826 DEGs up-regulated and 1 457 DEGs down-regulated significantly. All the DEGs were annotated by KEGG pathway to 31 metabolic pathways. About 2 049 differential expression proteins were detected by two-dimensional gel electrophoresis. After exposed to 30 mg / L Cu for 96 h,130 protein spots showed significant changes,among which 56 spots up-regulated and 74 spots down-regulated. The mass spectrometry analysis of part differential expression pro-teins showed that the resistant proteins were up-regulated in wheat roots,such as GSTs and 27K protein, but the expression of physiological metabolism related proteins down-regulated trend after being exposed to 30 mg / L Cu.