超高效液相色谱法测定荔枝蜜中黄酮类化合物

Determination of Favonoids in Litchi Honeys by UPLC

为建立超高效液相色谱-二极管阵列检测法（UPLC-PDA）分析荔枝蜜中黄酮类化合物,选用BEH C18（2.1 mm×50 mm,1.7μm）色谱柱,流动相A为0.05%的甲酸水、B为甲醇,进样量2μL,流速0.3 m L/min,柱温23℃,检测波长255 nm,建立了超高效液相色谱-二极管阵列检测法（UPLC-PDA）。结果显示,检测芦丁标准曲线的线性方程为y=21 436x-36 053,线性相关系数为0.996 2,变异系数为0.94%,加标回收率为97.36%~103.32%。8种荔枝蜜含有7种共有组分,其保留时间分别为0.993、2.801、3.141、4.365（该组分经确证为芦丁）、5.250、5.720、5.972 min。

To identify the characteristic components of litchi honey,the flavonoid in litchi honey by ultra performance liquid chromatography（ UPLC） coupled with photo-diode array （ PDA） was analyzed. The standard curve linear equation of the method for rutin detection was as follows,y = 21 436x - 36 053. The linear correlation coefficient was 0. 996 2 and the relative standard deviation of accuracy test was 0. 94% , the recovery of standard sample added was 97. 36% —103. 32% . The results showed that eight kinds of litchi honeys contained seven characteristic peaks in common. Their retention times were 0. 993 min, 2. 801 min,3. 141 min,4. 365 min （confirmed as rutin）,5. 250 min,5. 720 min and 5. 972 min, successively.