子宫内膜异位症患者腹腔液单个核细胞Fas的表达及意义

Expression of Fas in peritoneal fluid mononuclear cells among endometriosis patients and its significance

目的：探讨子宫内膜异位症与 Fas 介导的腹腔液单个核细胞凋亡的关系。方法选取手术后病理证实为子宫内膜异位症的患者55例作为子宫内膜异位症组，以非子宫内膜异位症患者52例作为对照组，分离腹腔液单个核细胞，应用实时逆转录聚合酶链反应（RT－PCR）测定 Fas mRNA 的表达水平，应用流式细胞术定量检测 Fas 表达细胞百分率。结果子宫内膜异位症组腹腔液单个核细胞 Fas mRNA 及 Fas 表达细胞百分率明显高于对照组（P ＜0．05），Fas mRNA 及 Fas 表达细胞百分率在子宫内膜异位症组Ⅰ～Ⅱ期与Ⅲ～Ⅳ期中表达差异无统计学意义（P ＞0．05）。结论子宫内膜异位症患者腹腔液单个核细胞 Fas 表达异常，对子宫内膜异位症具有一定的作用。

Objective To investigate the relationship of Fas expression in peritoneal fluid mononuclear cells and endometriosis.Methods A total of 55 patients with histopathologically diagnosed endometriosis after operation were enrolled as endometriosis group, and 52 patients without endometriosis were enrolled as control group.The peritoneal fluid mononuclear cells were isolated, the expression levels of Fas mRNA were determined by reverse transcriptase-polymerase chain reaction （RT-PCR）, and the percentage of Fas expression cells were quantitatively detected by flow cytometry.Results Fas mRNA and the percentage of Fas expression cells were significantly higher in endometriosis group than those in control group（P 〈0.05）.There was no statistical significance between stage Ⅰ-Ⅱand stage Ⅲ-Ⅳ（P 〉0.05）.Conclusions The abnormal Fas expression in peritoneal fluid mononuclear cells may play an important role in endometriosis.